Abstract: Embodiments of microfluidic systems and methods of manufacturing are described herein, which utilize an automated microfluidic plumbing technology with addressable ports capable of minimally disruptive additive and subtractive (including probing) cell and/or fluid manipulation at any desired location(s) within living cultures. The addressable microfluidic ports may be integrated throughout cell cultures in microfluidic systems for microfluidic tissue scaffolds, in two- or three-dimensional spatial arrangements. The addressable microfluidic ports may be used for controlling and/or monitoring cell behavior over time at different user-selected locations within cell cultures. Also provided are methods for fabricating such microfluidic devices and microfluidic tissue scaffolds.

Abstract: Embodiments of microfluidic systems and methods of manufacturing are described herein, which utilize an automated microfluidic plumbing technology with addressable ports capable of minimally disruptive additive and subtractive (including probing) cell and/or fluid manipulation at any desired location(s) within living cultures. The addressable microfluidic ports may be integrated throughout cell cultures in microfluidic systems for microfluidic tissue scaffolds, in two- or three-dimensional spatial arrangements. The addressable microfluidic ports may be used for controlling and/or monitoring cell behavior over time at different user-selected locations within cell cultures. Also provided are methods for fabricating such microfluidic devices and microfluidic tissue scaffolds.