Abstract: The present disclosure provides an anti-pollution consumable and a method for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) molecular diagnosis using the same, belonging to the technical field of nucleic acid detection and molecular diagnostics. The anti-pollution consumable includes an outer reaction tube, a sleeve and an inner reaction tube, where the inner reaction tube includes a second hollow cylindrical upper body and a second-type conical lower body sequentially from top to bottom; a top end of the second hollow cylindrical upper body is externally connected with a fixing ring perpendicular to the second hollow cylindrical upper body; a number of drain holes are provided at a bottom of the second-type conical lower body; the drain hole has a diameter of 0.01-0.8 mm; the drain hole is used for hydrophobic treatment; and the inner reaction tube is fixed inside the outer reaction tube through the sleeve.