Abstract: Methods and compositions for modifying the 3? untranslated region or coding sequence of endogenous genes using rare-cutting endonucleases and donor molecules. The methods and compositions described herein can be used to modify the coding sequence of endogenous genes or to facilitate early termination of transcripts.

Abstract: Methods and compositions for modifying the expression of endogenous genes or modifying the coding sequence of endogenous genes.

Abstract: Materials and methods are provided for making plants with altered levels of amino acids, particularly by making controlled frameshift mutations in genes that are highly expressed in plant leaves or plant seeds.

Abstract: Methods and compositions for modifying the coding sequence of endogenous genes using rare-cutting endonucleases. The methods and compositions described herein can be used to modify the endogenous USH2A gene.

Abstract: Methods and compositions for modifying the coding sequence of endogenous genes using rare-cutting endonucleases. The methods and compositions described herein can be used to modify the endogenous von Willebrand factor gene.

Abstract: Materials and methods are provided for making plants (e.g., Triticum varieties) with increased levels of dietary fiber, such as by making TALE nuclease-induced mutations in alleles encoding starch branching enzyme IIa (SBEIIa) and starch branching enzyme IIb (SBEIIb).

Abstract: Materials and methods for changing expression of a gene product in a plant are provided, and in an embodiment for creating herbicide tolerant plants are described herein. The methods provide for inserting into a plant genome, at a different locus than an endogenous gene, a genomic or coding sequence of the gene, which may be modified, into a genetic location that is different from the endogenous gene and where there is a desired transcriptional activity. The methods described herein can include the targeted insertion of an endogenous 5-enolpyruvylshikimate-3-phosphate synthase gene into a genomic locus that enables sufficient expression to confer herbicide tolerance.

Abstract: Materials and methods are provided for making plants with altered levels of amino acids, particularly by making controlled frameshift mutations in genes that are highly expressed in plant leaves or plant seeds.

Abstract: Systems and methods for gene targeting in plants, including systems and methods that include the use of geminiviruses and customizable endonucleases.