Abstract: The instant disclosure is directed to methods for generating stem cell-derived immune cells with enhanced function. Disclosed herein are methods for modifying a stem or progenitor cell capable of differentiating into an immune cell to inhibit the function of at least one gene selected from DGK? and DGK?, and directing differentiation of that stem or progenitor cells towards enhanced immune cells. Also disclosed herein are immune cells or stem cells made by the present methods, as well as the use of immune cells in therapeutic treatment.

Abstract: This disclosure relates to methods for producing immune cells with enhanced function. More specifically, disclosed herein is a method for enhancing the function of an immune cell comprising modifying an immune cell to inhibit the function of at least one gene selected from the group consisting of RC3H1, RC3H2, A2AR, FAS, TGFBR1, and TGFBR2. Also disclosed herein is a method comprising modifying a stem or progenitor cell capable of differentiating into an immune cell to inhibit the function of at least one gene selected from the group consisting of RC3H1, RC3H2, A2AR, FAS, TGFBR1, and TGFBR2. Also disclosed herein are immune cells or stem cells made by the present methods, as well as the use of immune cells in therapeutic treatment.

Abstract: Systems and methods for determining a sequence of at least a portion of a target polymer from a subject are provided. A dataset that comprises one or more image files is obtained. A combined plurality of localizations based at least in part on each respective plurality of fluorophore localizations is determined for each image file in the one or more image files. Each localization in the combined plurality of localizations includes a target polymer position identity and a spatial location. The plurality of localizations are segmented into one or more target polymer strands. Each target polymer strand corresponds to a respective subset of localizations and target polymer position identities. A respective target polymer sequence is assembled using each subset of localizations for each target polymer strand, thereby providing a set of target polymer sequences.

Abstract: This disclosure relates to immune cells (such as T cells or NK cells) modified in their cell surface receptors to recognize one or more target antigens, in particular tumor-associated antigens. This disclosure also relates to a simple method for editing cell receptors, in particular cell surface receptors naturally expressed by immune cells such as T cells or NK cells, to create modified immune cells (e.g., cytotoxic cells) targeted against one or more target antigens, in particular tumor-associated antigens. Further, this disclosure relates to stem cells modified in one or more endogenous genes encoding one or more cell surface receptors and capable of differentiating into immune cells expressing modified cell surface receptors that recognize one or more target antigens. In addition, this disclosure relates to methods of making such modified stem cells.

Abstract: A system and method for resolving data through a probabilistic data structure can include initializing a B-field data structure, inserting a key-value element into the B-field data structure, selecting at least one key query, and looking up the value of a key lookup request through the B-field data structure.

Abstract: Systems and methods for determining a sequence of at least a portion of a target polymer from a subject are provided. A dataset that comprises one or more image files is obtained. A combined plurality of localizations based at least in part on each respective plurality of fluorophore localizations is determined for each image file in the one or more image files. Each localization in the combined plurality of localizations includes a target polymer position identity and a spatial location. The plurality of localizations are segmented into one or more target polymer strands. Each target polymer strand corresponds to a respective subset of localizations and target polymer position identities. A respective target polymer sequence is assembled using each subset of localizations for each target polymer strand, thereby providing a set of target polymer sequences.

Abstract: A system and method for resolving data through a probabilistic data structure can include initializing a B-field data structure, inserting a key-value element into the B-field data structure, selecting at least one key query, and looking up the value of a key lookup request through the B-field data structure.

Abstract: A system and method for resolving data through a probabilistic data structure can include initializing a B-field data structure, inserting a key-value element into the B-field data structure, selecting at least one key query, and looking up the value of a key lookup request through the B-field data structure.

Abstract: A system and method for resolving data through a probabilistic data structure can include initializing a B-field data structure, inserting a key-value element into the B-field data structure, selecting at least one key query, and looking up the value of a key lookup request through the B-field data structure.

Abstract: A water wave-based energy conversion system comprising: at least one buoyant body capable of floating on or below the surface of a body of water; at least one redirection device located at a stationary position; an energy converter; and at least one elongate connector operatively coupling the at least one buoyant body to the energy converter through the at least one redirection device. In use, the elongate connector transfers motion of the at least one buoyant body resulting from waves in the body of water to the energy converter for conversion into useful energy forms or products.