Abstract: Disclosed herein are new immunotherapy methods that involve the isolation of cancer stem cells from tumor tissue and use of the cells either directly or indirectly through proteins or other factors associated with the cells to activate antigen presenting cells. The activated antigen presenting cells are useful as a therapy against the tumor. Also disclosed herein are novel methods of isolating and characterizing cancer stem cells and producing individual cancer stem cell lines. Dendritic cell lines are also disclosed herein.