Patents by Inventor Nicholas Heredia
Nicholas Heredia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240124927Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.Type: ApplicationFiled: January 3, 2024Publication date: April 18, 2024Inventors: Nicholas HEREDIA, Dianna MAAR
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Patent number: 11898201Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.Type: GrantFiled: January 13, 2021Date of Patent: February 13, 2024Assignee: Bio-Rad Laboratories, Inc.Inventors: Nicholas Heredia, Dianna Maar
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Publication number: 20230390726Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: ApplicationFiled: August 11, 2023Publication date: December 7, 2023Inventors: Ronald LEBOFSKY, Nicholas HEREDIA
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Patent number: 11759761Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: GrantFiled: July 20, 2021Date of Patent: September 19, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Ronald Lebofsky, Nicholas Heredia
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Publication number: 20220040663Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: ApplicationFiled: July 20, 2021Publication date: February 10, 2022Inventors: Ronald LEBOFSKY, Nicholas HEREDIA
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Patent number: 11097247Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: GrantFiled: June 25, 2020Date of Patent: August 24, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Ronald Lebofsky, Nicholas Heredia
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Publication number: 20210207208Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.Type: ApplicationFiled: January 13, 2021Publication date: July 8, 2021Inventors: Nicholas HEREDIA, Dianna MAAR
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Patent number: 10920270Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.Type: GrantFiled: March 22, 2019Date of Patent: February 16, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Nicholas Heredia, Dianna Maar
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Publication number: 20200324264Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: ApplicationFiled: June 25, 2020Publication date: October 15, 2020Inventors: Ronald LEBOFSKY, Nicholas HEREDIA
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Patent number: 10730030Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: GrantFiled: January 6, 2017Date of Patent: August 4, 2020Assignee: Bio-Rad Laboratories, Inc.Inventors: Ronald Lebofsky, Nicholas Heredia
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Publication number: 20190211388Abstract: Methods and compositions are provided for improved nucleic acid amplification assays. In some embodiments, the nucleic acid amplification assay is a tagged amplicon primer extension (TAPE) nucleic acid amplification reaction.Type: ApplicationFiled: March 22, 2019Publication date: July 11, 2019Inventors: Nicholas HEREDIA, Dianna MAAR
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Patent number: 10280455Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.Type: GrantFiled: December 28, 2016Date of Patent: May 7, 2019Assignee: Bio-Rad Laboratories, Inc.Inventors: Nicholas Heredia, Dianna Maar
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Publication number: 20170232417Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: ApplicationFiled: January 6, 2017Publication date: August 17, 2017Inventors: Ronald Lebofsky, Nicholas Heredia
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Publication number: 20170191127Abstract: Methods of preparing a target gene-enriched library are provided. In one aspect, the method comprises partitioning polynucleotide fragments into a plurality of partitions, wherein each partition further comprises a plurality of primer pairs for amplifying a target gene and wherein the primers comprise a portion of an adapter sequence; amplifying a target gene sequence to generate an amplicon comprising the target gene sequence flanked on either end by a portion of an adapter sequence; purifying the amplicon; and amplifying the amplicon using primers comprising full-length adapter sequences.Type: ApplicationFiled: December 29, 2016Publication date: July 6, 2017Inventors: Shawn Hodges, Nicholas Heredia
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Publication number: 20170191124Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.Type: ApplicationFiled: December 28, 2016Publication date: July 6, 2017Inventors: Nicholas Heredia, Dianna Maar