Abstract: Methods for gene targeting or targeted insertion in cells. The methods and compositions described herein can be used to identify the relative frequency of donor molecule integration.

Abstract: Methods and compositions for modifying the coding sequence of endogenous genes using rare-cutting endonucleases and transposases. The methods and compositions described herein can be used to modify the coding sequence of endogenous genes.

Abstract: The present disclosure is in the field of medical devices and gene editing, particularly the use of medical devices for the targeted delivery of gene editing reagents in vivo or ex vivo. The methods and materials described herein provide control over the location and timing of delivery, along with the ability to deliver gene editing reagents as nucleic acids, virus particles, or protein. Furthermore, the methods and materials can be used to reduce or eliminate the systemic spread of gene editing reagents in non-target tissues/organs. The methods and devices described herein can be used for gene editing in animals.

Abstract: Materials and methods for creating plants (e.g., alfalfa lines) with reduced lignin content and composition are provided herein, as are plants, plant parts, and plant cells generated by the methods provided herein.

Abstract: Materials and methods are provided for making plants (e.g., soybean varieties, wheat varieties, or corn varieties) with altered amino acid content. For example, materials and methods are provided for making TALE nuclease-induced mutations in genes encoding seed storage proteins, or by making TALE nuclease-induced deletions of within seed storage protein genes.

Abstract: Materials and methods for high efficiency gene targeting at multiple genomic sites in a single cell are provided herein.

Abstract: Materials and methods for conferring geminivirus resistance to plants, and particularly to materials and methods for using CRISPR/Cas systems to confer resistance to geminiviruses to plants.

Abstract: Methods for genome engineering, including methods utilizing transient expression of a nuclease utilizing modified transfer-DNA (T-DNA) plasmids, are provided herein.

Abstract: Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein.

Abstract: Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein.