Patents by Inventor Nicholas J. DePolo

Nicholas J. DePolo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia and other disorders
    Patent number: 6818439
    Abstract: Methods are provided for obtaining measurable levels of a protein, nucleic acid molecule, or enzymatic product in a bodily fluid or cells of a human, comprising the step of administering to a human a recombinant retroviral preparation having a titer on HT1080 cells of greater than 105 cfu/ml, wherein the recombinant retroviral preparation is capable of directing the expression of a protein, nucleic acid molecule, or enzyme which generates an enzymatic product, such that measurable levels of the protein, nucleic acid molecule, or enzymatic product may be obtained in the bodily fluid or cells of the human.
    Type: Grant
    Filed: December 30, 1997
    Date of Patent: November 16, 2004
    Assignee: Chiron Corporation
    Inventors: Douglas J. Jolly, Stephen Chang, James G. Respess, Nicholas J. DePolo, David Chi-Tang Hsu, Carlos E. Ibanez, Judith Greengard, Lee Will
  • Methods for producing a vector producing cell line utilizing a high multiplicity of transduction
    Patent number: 6410316
    Abstract: Retroviral vector particle producing cells are provided, wherein the cell (a) has greater than 5 stably integrated copies of a retroviral vector construct; (b) produces greater than 10 infectious recombinant retroviral vector particles per cell per day; and (c) produces replication incompetent retroviral vector particles.
    Type: Grant
    Filed: March 15, 2000
    Date of Patent: June 25, 2002
    Assignee: Chiron Corporation
    Inventors: Philip L. Sheridan, Mordechai Bodner, Nicholas J. DePolo, Sybille Sauter, Stephen M. W. Chang
  • Crossless retroviral vectors
    Patent number: 6333195
    Abstract: Retroviral vector constructs are described which have a 5′ LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3′ LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.
    Type: Grant
    Filed: January 7, 2000
    Date of Patent: December 25, 2001
    Assignee: Chiron Corporation
    Inventors: James G. Respess, Nicholas J. DePolo, Sunil Chada, Sybille Sauter, Mordechai Bodner, David A. Driver
  • Crossless retroviral vectors
    Patent number: 6013517
    Abstract: Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression-cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.
    Type: Grant
    Filed: May 5, 1997
    Date of Patent: January 11, 2000
    Assignee: Chiron Corporation
    Inventors: James G. Respess, Nicholas J. DePolo, Sunil Chada, Sybille Sauter, Mordechai Bodner, David A. Driver