Abstract: Disclosed herein are compositions, kits and methods for determining the concentration of fluid-phase MASP-2/C1-INH complex in a biological fluid, such as a biological fluid obtained from a subject infected with SARS-CoV-2. Also disclosed are methods of using said compositions, methods and kits for detection of MASP-2/C1-INH complex to determine the status of lectin pathway activation in a mammalian subject and thereby assess the risk of a subject that is or has been infected with SARS-CoV-2 for developing COVID-19-related ARDS or other poor outcome, or determine the need for treatment or efficacy of treatment of a subject in need thereof with a complement inhibitor such as a MASP-2 inhibitory agent.

Abstract: A service provider network includes a certificate manager that auto-generates and auto-renews security certificates for customers of the provider network. The security certificates may be usable to implement a Secure Sockets Layer (SSL) protocol, or other types of security protocols. The certificate manager generates a public key, private key pair for the customer, generates the certificate signing request (CSR) on behalf of the customer, transmits the CSR to the certificate authority (CA), and binds the resulting CA-generated certificate and private key to whatever internet-facing service the customer chooses (e.g., a load balancer).