Abstract: This invention relates to enzyme-sensitive biosensors and methods and kits using the same. Specifically, the invention relates to methods, systems and kits for the detection of enzymes using the chemical shift observed in an isotope complexed to the biosensor resulting from a change in the biosensor as the result of the enzyme's activity.

Abstract: This invention relates to enzyme-sensitive biosensors and methods and kits using the same. Specifically, the invention relates to methods, systems and kits for the detection of enzymes using the chemical shift observed in an isotope complexed to the biosensor resulting from a change in the biosensor as the result of the enzyme's activity.

Abstract: The present invention provides a method and apparatus for increasing the intensity of coherent population trapping (CPT) resonances, used in atomic clocks and magnetometers, by pumping the atoms with light of alternating polarization. Pumping with such light, characterized by a photon spin vector that alternates in direction at a hyperfine frequency of the atoms at the location of the atoms, is referred to as push-pull pumping. In one embodiment of the system of the present invention, alkali-metal vapor is pumped with alternating circular-polarization D1 laser light that is intensity modulated at appropriate resonance frequencies, thereby exciting CPT resonances, which can be observed as increase in the mean transmittance of the alkali-metal vapor. These resonances are substantially enhanced due to an optically-induced concentration of atoms in the resonant energy sublevels.