Abstract: In certain embodiments, the present invention provides methods to prepare insulin secreting cells from a skin sample from a mammal.

Abstract: A bioprinter and methods of using the bioprinter. The bioprinter permits selective three-dimensional movement of multiple nozzle assemblies during operation of the bioprinter. The bioprinter can be used to produce scaffold-free tissue constructs having a plurality of cellular elements interspersed among a semi-permeable vascular network. The bioprinter can also be used to print a tissue construct directly onto a tissue defect of a subject. The bioprinter can be provided as part of a bioprinting system that includes a scanner for imaging the tissue defect.

Abstract: Embodiments of the invention include dimeric soluble MHC molecules (dsMHCs) and compositions and methods for their use. The molecules may be used to downregulate activated T cells. The dsMHCs may be administered by intraperitoneal infusion into a tissue or an organ recipient. The administration of dsMHCs molecules may also be supplemented with immunosupressant to induce the engraftment of allografts. dsMHCs molecules may also be used for visualization of alloreactive T cells in various tissues or organs.