Abstract: This invention relates to a method for lysing cells. The method comprises simultaneously flowing a cell suspension and a lysis solution through a static mixer, wherein the cells exit the static mixer lysed. In another aspect of the present invention, the invention relates to a method for precipitating cell components, protein, and nucleic acids from a cell lysate or other solution containing precipitable material. The method comprises simultaneously flowing a cell lysate or other protein containing solution and a precipitating solution through a static mixer, wherein the lysate or protein solution exits the static mixer with its precipitable components precipitated. In another aspect of the present invention, the invention relates to a method where the two above-mentioned methods above are combined by using static mixers in series.

Abstract: This invention relates to a serum-free eukaryotic cell culture medium supplement. The supplement comprises carbon sources, vitamins, inorganic salts, amino acids and a protein digest.The medium supplement of the present invention enables the maintenance of mammalian cell cultures at cell densities equal to or greater than that obtained with batch culture methods while increasing longevity and productivity.

Abstract: This invention relates to a serum-free eukaryotic cell culture medium supplement. The supplement comprises carbon sources, vitamins, inorganic salts, amino acids and a protein digest. The medium supplement of the present invention enables the maintenance of mammalian cell cultures at cell densities equal to or greater than that obtained with batch culture methods while increasing longevity and productivity.

Abstract: A variant strain of Agrobacter radiobacter has been found to produce a unique heteropolysaccharide containing D-galactose, D-glucose and pyruvic acid in approximate molar ratios of 2:15:2 in a seventeen member repeat unit having the structure shown below, wherein Glc represents D-glucose, Gal represents D-galactose and Pyr represents pyruvic acid, with the numerals indicating the positions of linkage in these ring structures. The heteropolysaccharide is useful in mobility control in oil reservoir formations, especially when crosslinked with trivalent metal cations (Cr.sup.3+, Al.sup.3+, Fe.sup.3+ or Ti.sup.3+) to form a gel which preferentially fills high permeability zones in such reservoirs.