Abstract: A method of treating at least one surface includes a step of contacting the at least one surface with an aqueous solution obtained by mixing in water an aqueous formulation having at least one biosurfactant and at least one organic acid. The weight ratio between the at least one biosurfactant and the at least one organic acid is between 1:1000 and 10:1, in an amount such that the aqueous solution includes the at least one biosurfactant in an amount between 0.0001 and 10% by weight and the at least one organic acid in an amount between 0.001 and 10% by weight. The at least one biosurfactant is selected from glycolipids, lipopeptides, lipoproteins, phospholipids and polymeric biosurfactants. The at least one organic acid is selected from citric acid, lactic acid, acetic acid, malic acid, maleic acid, formic acid, caprylic acid, methanesulfonic acid, tartaric acid, succinic acid, and polyaspartic acid.