Abstract: Methods are provided for the large-scale high-content analysis of biological samples. In some embodiments, the methods are implemented in a reversed open microwell system that includes an array of open microwells, at least one microchannel, at least one input port and at least one output port. In certain embodiments, the reversed open microwell system can be inserted in an automated management system which includes an incubator at controlled temperature, humidity and CO2 levels, a fluid dispensing system, and is capable of phase-contrast and fluorescence image acquisition. Also provided are kits for introducing fluids into a microfluidic device, and systems for discharging one or more fluids in a microfluidic device.

Abstract: A method for analysis of biological samples, implemented in a reversed open microwell system which includes an array of open microwells, a microchannel, an input port for reagents and/or biological samples and an output port. The ports are in microfluidic communication with the microchannel. The microchannel has a cross-section area of micrometric dimensions and provides fluid to the microwells. The microwell system is inserted in an automated management system that includes: an incubator at controlled temperature, humidity and CO2, fluid dispensing system, phase-contrast and fluorescence image acquisition. A kit introduces fluids in a microfluidic device that includes a tip. A microfluidic device and system include a microchannel and an input region having a vertical channel. The tip and the vertical channel are dimensioned to produce an interference coupling. A discharge region includes: a discharge container connected with the microfluidic device through a discharge channel and an output port.

Abstract: Methods are provided for the large-scale high-content analysis of biological samples. In some embodiments, the methods are implemented in a reversed open microwell system that includes an array of open microwells, at least one microchannel, at least one input port and at least one output port In certain embodiments, the reversed open microwell system can be inserted in an automated management system which includes an incubator at controlled temperature, humidity and CO2 levels, a fluid dispensing system, and is capable of phase-contrast and fluorescence image acquisition. Also provided are kits for introducing fluids into a microfluidic device and systems for discharging one or more fluids in a microfluidic device.