Patents by Inventor Nicolaas M. J. Vermeulen

Nicolaas M. J. Vermeulen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Minor groove binder (MGB)-oligonucleotide miRNA antagonists
    Patent number: 9334495
    Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.
    Type: Grant
    Filed: November 23, 2010
    Date of Patent: May 10, 2016
    Assignee: ELITECHGROUP B.V.
    Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M. J. Vermeulen, Walt Mahoney
  • Minor groove binder (MGB)-oligonucleotide miRNA antagonists
    Patent number: 8980855
    Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.
    Type: Grant
    Filed: June 7, 2012
    Date of Patent: March 17, 2015
    Assignees: Elitech Holding B.V., GE Healthcare Dharmacon, Inc.
    Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M. J. Vermeulen, Walt Mahoney
  • Compounds and methods for fluorescent labeling
    Patent number: 8569516
    Abstract: Compounds useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: February 17, 2006
    Date of Patent: October 29, 2013
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • MINOR GROOVE BINDER (MGB)-OLIGONUCLEOTIDE MIRNA ANTAGONISTS
    Publication number: 20120245219
    Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.
    Type: Application
    Filed: June 7, 2012
    Publication date: September 27, 2012
    Applicants: DHARMACON, INC., ELITECH HOLDING B.V.
    Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M.J. Vermeulen, Walt Mahoney
  • Amplification methods
    Patent number: 8067177
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: November 29, 2011
    Assignee: Epoch Biosciences, Inc.
    Inventors: Walt Mahoney, Nicolaas M. J. Vermeulen, Irina Afonina
  • MINOR GROOVE BINDER (MGB)-OLIGONUCLEOTIDE MIRNA ANTAGONISTS
    Publication number: 20110172289
    Abstract: Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders (“MGBs”). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.
    Type: Application
    Filed: November 23, 2010
    Publication date: July 14, 2011
    Inventors: Anastasia Khvorova, Annaleen Vermeulen, Rob Kaiser, Jon Karpilow, Nicolaas M.J. Vermeulen, Walt Mahoney
  • Fluorescent quenching detecting reagents and methods
    Patent number: 7897736
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Grant
    Filed: January 6, 2010
    Date of Patent: March 1, 2011
    Assignee: Elitech Holding B.V.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M. J. Vermeulen
  • AMPLIFICATION METHODS
    Publication number: 20110027780
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Application
    Filed: March 17, 2010
    Publication date: February 3, 2011
    Inventors: Walt Mahoney, Nicolaas M.J. Vermeulen, Irina Afonina
  • Negatively charged minor groove binders
    Patent number: 7851606
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: December 14, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Eugeny A. Lukhtanov, Nicolaas M. J. Vermeulen, Alexander A. Gall
  • FLUORESCENT QUENCHING DETECTING REAGENTS AND METHODS
    Publication number: 20100174058
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: January 6, 2010
    Publication date: July 8, 2010
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
  • TM leveling methods
    Patent number: 7751982
    Abstract: Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 6, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • NEGATIVELY CHARGED MINOR GROOVE BINDERS
    Publication number: 20100137614
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Application
    Filed: August 14, 2009
    Publication date: June 3, 2010
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Nicolaas M.J. Vermeulen, Alexander A. Gall
  • Amplification methods
    Patent number: 7723038
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: May 25, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Walt Mahoney, Nicolaas M. J. Vermeulen, Irina Afonina
  • Systems and methods for predicting oligonucleotide melting temperature (TS)
    Patent number: 7715989
    Abstract: Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: June 18, 2002
    Date of Patent: May 11, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Karen M. Singer, legal representative, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Fluorescent quenching detection reagents and methods
    Patent number: 7662942
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Grant
    Filed: January 23, 2006
    Date of Patent: February 16, 2010
    Assignee: Elitech Holdings, B.V.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M. J. Vermeulen
  • Compounds and methods for fluorescent labeling
    Patent number: 7601851
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: May 10, 2005
    Date of Patent: October 13, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Negatively charged minor groove binders
    Patent number: 7582739
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Grant
    Filed: March 11, 2003
    Date of Patent: September 1, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Nicolaas M. J. Vermeulen, Alexander A. Gall
  • AMPLIFICATION METHODS
    Publication number: 20080248474
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Application
    Filed: December 18, 2007
    Publication date: October 9, 2008
    Applicant: EPOCH BIOSCIENCES, INC
    Inventors: WALT MAHONEY, Nicolaas M.J. Vermeulen, Irina Afonina
  • Tleveling compositions
    Patent number: 7368549
    Abstract: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: April 19, 2005
    Date of Patent: May 6, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Robert O. Dempcy, Alexander A Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Amplification methods
    Patent number: 7319022
    Abstract: Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe.
    Type: Grant
    Filed: August 11, 2005
    Date of Patent: January 15, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Walt Mahoney, Nicolaas M. J. Vermeulen, Irina Afonina