Patents by Inventor Nicolas C. Pegard

Nicolas C. Pegard has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11592654
    Abstract: Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.
    Type: Grant
    Filed: January 20, 2021
    Date of Patent: February 28, 2023
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Laura Waller, Hillel Adesnik, Nicolas C. Pégard
  • Publication number: 20210239956
    Abstract: Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.
    Type: Application
    Filed: January 20, 2021
    Publication date: August 5, 2021
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Laura Waller, Hillel Adesnik, Nicolas C. Pégard
  • Patent number: 10935776
    Abstract: Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR 1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: March 2, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Laura Waller, Hillel Adesnik, Nicolas C. Pégard
  • Publication number: 20190227490
    Abstract: Apparatus and methods for 3D-Scanless Holographic Optogenetics with Temporal focusing (3D-SHOT), which allows precise, simultaneous photo-activation of arbitrary sets of neurons anywhere within the addressable volume of the microscope. Soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR 1, optimized for multiphoton activation and suppression are also provided. The methods use point-cloud holography to place multiple copies of a temporally focused disc matching the dimensions of a designated neuron's cell body. Experiments in cultured cells, brain slices, and in living mice demonstrate single-neuron spatial resolution even when optically targeting randomly distributed groups of neurons in 3D.
    Type: Application
    Filed: January 23, 2019
    Publication date: July 25, 2019
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Laura Waller, Hillel Adesnik, Nicolas C. Pégard
  • Patent number: 10048201
    Abstract: A microscope is disclosed, the microscope having a light source defining an optical axis along a Z direction and a detector disposed in X-Y direction, orthogonal to the optical axis, the detector configured to capture images of an object. The microscope includes a fluid channel having an inlet and an outlet configured with a fluid flow to transport the object from the inlet to the outlet. The detector is configured to capture a plurality of images of the object as the object moves from the inlet to the outlet. The plurality of images of the object may have different heights of the sample with respect to the detector as the sample flows through the channel. The channel may be tilted with respect to the optical axis. The detector may be tilted with respect to the optical axis.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: August 14, 2018
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Jason W. Fleischer, Nicolas C. Pegard