Abstract: A focus adjustment method for acquiring an image of a surface of interest of a sample by a holographic imager includes the steps of: placing the sample including at least one reference object having a known shape and described by characterising parameters having at least position parameters acquiring an image and determining the position of the reference object with respect to the acquisition plane, by applying a light diffraction model involving the spatial parameters of the reference object estimated by approximating the appearance of the reference object in the holographic image acquired, and determining the position of the surface of interest with respect to the acquisition plane from a position of the reference object and focus adjustment of the image acquisition.

Abstract: The present invention relates to a method for predicting the susceptibility of a microbial strain to an antimicrobial agent, the method being characterized in that it comprises implementation, by data-processing means (4) of a client (2), of steps of: (a) obtaining a hyperspectral image between 390 nm and 900 nm representing at least one colony of said strain in a sample devoid of antimicrobial agent (22); (b) determining a spectrum of the colony based on pixels of said hyperspectral image corresponding to said colony, “test spectrum” below; (c) comparing said test spectrum with microbial classes of a database of predetermined data, “reference microbial class” below, said classes corresponding to a taxonomic level lower than species and being learnt using at least one hyperspectral spectrum of a microbial strain, the database containing, for each reference microbial class, the susceptibility to the microbial agent of the reference microbial class; (d) determining the susceptibility of the microbial strain to

Abstract: A focus adjustment method for acquiring an image of a surface of interest of a sample by a holographic imager includes the steps of: placing the sample including at least one reference object having a known shape and described by characterising parameters having at least position parameters acquiring an image and determining the position of the reference object with respect to the acquisition plane, by applying a light diffraction model involving the spatial parameters of the reference object estimated by approximating the appearance of the reference object in the holographic image acquired, and determining the position of the surface of interest with respect to the acquisition plane from a position of the reference object and focus adjustment of the image acquisition.