Abstract: The present invention relates to mutant bacteria of the genus Clostridium beijerinckii, CNCM I-4985, CNCM I-4986, CNCM I-4987 and CNCM I-4988, deposited at the Institut Pasteur (CNCM, 25 rue du Docteur Roux, F-75724 PARIS Cedex 15, France) on 27 May 2015, and CNCM I-5027, CNCM I-5028 and CNCM I-5029 deposited at the Institut Pasteur (CNCM, 25 rue du Docteur Roux, F-75724 PARIS Cedex 15, France) on 26 Nov. 2015, which are useful in the fermentation of sugars for the production of isopropanol and butanol.

Abstract: The present invention concerns a process for the production of an aqueous mixture comprising isopropanol, n-butanol and ethanol, comprising a step in which an aqueous solution containing C5 and/or C6 sugars and acetate is fermented under strict anaerobic conditions in the presence of a fermenting microorganism of the genus Clostridium and in which said aqueous solution has an acetate concentration in the range 0.5 to 10 g/L and a ratio by weight of (acetate)/(sum of C5 and/or C6 sugars) in the range 0.005 to 0.35 g/g.

Abstract: The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.

Abstract: The invention relates to a multi-enzymatic preparation containing the secretome of the CNCM I-4639 strain of Aspergillus japonicus. This secretome, which contains in particular cellulases and hemicellulases, can be used for the saccharification of lignocellulosic substrates, in particular in combination with the secretome of Trichoderma reesei.

Abstract: The present invention relates to a method of producing sweet juice from lignocellulosic substrate, wherein enzymatic hydrolysis is carried out by performing cellulase supplementation with supported ?-glucosidases used in a reactor separate from the lignocellulose enzymatic hydrolysis reactor.

Abstract: The present invention describes the use of a genetically improved Trichoderma reesei strain allowing to limit microbiological contaminations during an industrial process. The genetic strain improvement allows the latter to overexpress an extracellular protein having known antimicrobial properties and compatible with the secretory system of strains of fungi such as Trichoderma reesei. This modified strain can be used to produce the cellulolytic and/or hemicellulolytic enzymes used in a method of producing ethanol from cellulosic or lignocellulosic materials referred to as “second generation” materials.

Abstract: The object of the present invention are novel fusion proteins comprising enzymes degrading plant cell walls, and the use thereof in a method of producing ethanol from lignocellulosic biomass.

Abstract: The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.

Abstract: The present invention relates to a method of producing sweet juice from lignocellulosic substrate, wherein enzymatic hydrolysis is carried out by performing cellulase supplementation with supported ?-glucosidases used in a reactor separate from the lignocellulose enzymatic hydrolysis reactor.

Abstract: The present invention describes the use of a genetically improved Trichoderma reesei strain allowing to limit microbiological contaminations during an industrial process. The genetic strain improvement allows the latter to overexpress an extracellular protein having known antimicrobial properties and compatible with the secretory system of strains of fungi such as Trichoderma reesei. This modified strain can be used to produce the cellulolytic and/or hemicellulolytic enzymes used in a method of producing ethanol from cellulosic or lignocellulosic materials referred to as “second generation” materials.

Abstract: The invention concerns an isolated or purified polypeptide having an activity in the MTBE degradation path, and/or at least one of the catabolic intermediates of MTBE, preferably selected from the group consisting of tert-butyl alcohol (TBA), 2-methyl 1,2-propanediol (2-M1, 2-PD), hydroxyisobutyraldehyde, hydroxyiso-butyric acid (HIBA), said polypeptide being selected from the group consisting of: a) a polypeptide comprising a sequence of amino acids selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 or SEQ ID NO:10; b) a polypeptide comprising a sequence of amino acids having at least 70% identity, preferably 75%, 80%, 90%, 95%, 98% or 99% with the sequence of amino acids of a polypeptide as defined in a); a polypeptide as defined in a) or b) whereof the sequence of amino acids comprises a substitution, deletion, insertion, addition or mutation of one or several amino acids over its entire length.