Abstract: Disclosed is a lignin that is soluble in a medium having a pH greater than or equal to 4, a solution containing same, a method for producing same, and uses thereof, in particular for preparing lignin fibers and carbon fibers. Soluble lignin may be obtained by enzymatic modification.

Abstract: A miniaturized electrochemical cell and a method for making it are provided. The method includes preparing at least one inner electrode of an electron conducting or semi-conducting material M1; providing a hollow support made of an electrically insulating material M6 and having at least one internal hollow channel; depositing on the external surface of the support a layer of an electrically conducting material M2; forming a template of colloidal particles of an electrically insulating material M3, on the M2 layer; depositing a layer of an electrically conducting material M4 on the M2 layer; depositing a layer L1 of an electron conducting or semi-conducting material M5 on the M4 layer, introducing the at least one inner electrode into the at least one internal hollow channel of the obtained structure; stabilizing the structure at its two open ends with an electrically insulating material M7; and removing M2, M3, M4 and M6 materials.

Abstract: A miniaturized electrochemical cell and a method for making it are provided. The method includes preparing at least one inner electrode of an electron conducting or semi-conducting material M1; providing a hollow support made of an electrically insulating material M6 and having at least one internal hollow channel; depositing on the external surface of the support a layer of an electrically conducting material M2; forming a template of colloidal particles of an electrically insulating material M3, on the M2 layer; depositing a layer of an electrically conducting material M4 on the M2 layer; depositing a layer L1 of an electron conducting or semi-conducting material M5 on the M4 layer, introducing the at least one inner electrode into the at least one internal hollow channel of the obtained structure; stabilizing the structure at its two open ends with an electrically insulating material M7; and removing M2, M3, M4 and M6 materials.

Abstract: A method for manufacturing a miniaturized electrochemical cell and a miniaturized electrochemical cell is provided. The method includes the following steps: a) forming a colloidal template of colloidal particles made of an electrically insulating material, on a substrate made of an electrically conducting material, b) depositing by electrodeposition in the void spaces of the colloidal template, at least three alternating layers forming a repeating unit, the alternating layers being made of an electron conducting material or a semi -conducting material, the intermediate layer(s) being made of a material M3 different from materials M1 and M2 constituting respectively the upper and lower layers, the material M3 having a standard potential lower than the standard potentials of the materials M1 and M2, c) removal of the material M3 of intermediate layer(s), and d) removal of the colloidal particles of the upper and lower layers to obtain the desired electrodes.

Abstract: The present invention relates to a novel Bacillus pumilus bilirubin oxidase, to the method for preparing same and also to the use thereof in particular for assaying bilirubin and for using enzymatic biofuel cells.

Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol) diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

Abstract: The present invention relates to a novel bilirubin oxidase from Magnaporthe oryzae, to the method of preparation thereof as well as use thereof notably for assay of bilirubin and for the application of enzymatic biofuel cells.

Abstract: The present invention relates to mutants of the Penicillium amagasakiense glucose oxidase (GOx) enzyme which are of use for assaying glucose and to the development in particular of glucose electrodes and of biocells which use glucose as fuel.

Abstract: A porous electrochemical electrode is made up of a solid cellular material provided in the form of a semi-graphitized carbon monolith comprising a hierarchized porous network free of mesopores and including macropores with a mean dimension dA of 1 ?m to 100 ?m, and micropores with a mean dimension dI of 0.5 nm to 2 nm, said macropores and micropores being interconnected. In said electrode, the macropores contain at least one electroactive species in direct contact with the semi-graphitized carbon that makes up the surface of the macropores. The invention also relates to a method for preparing such an electrode as well as to the use thereof as a biosensor or for manufacturing a biopile.

Abstract: The present invention relates to a novel Bacillus pumilus bilirubin oxidase, to the method for preparing same and also to the use thereof in particular for assaying bilirubin and for using enzymatic biofuel cells.

Abstract: The present invention relates to mutants of the Penicillium amagasakiense glucose oxidase (GOx) enzyme which are of use for assaying glucose and to the development in particular of glucose electrodes and of biocells which use glucose as fuel.

Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

Abstract: The present invention relates to novel mutants of PQQ s-GDH containing an amino acid substitution in position 428 of the protein sequence of the wild type PQQ s-GDH of Acinetobacter calcoaceticus (SEQ.ID. NO:2). The invention also relates to the use of said PQQ s-GDH mutants for the development of glucose electrodes of interest in the assay of glucose, in particular of blood glucose in diabetic subjects, and for implementing biofuel cells that utilize glucose as fuel.

Abstract: The present invention relates to a novel bilirubin oxidase from Magnaporthe oryzae, to the method of preparation thereof as well as use thereof notably for assay of bilirubin and for the application of enzymatic biofuel cells.

Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol) diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

Abstract: A porous electrochemical electrode is made up of a solid cellular material provided in the form of a semi-graphitised carbon monolith comprising a hierarchised porous network free of mesopores and including macropores with a mean dimension dA of 1 ?m to 100 ?m, and micropores with a mean dimension dI of 0.5 nm to 2 nm, said macropores and micropores being interconnected. In said electrode, the macropores contain at least one electroactive species in direct contact with the semi-graphitised carbon that makes up the surface of the macropores. The invention also relates to a method for preparing such an electrode as well as to the use thereof as a biosensor or for manufacturing a biopile.

Abstract: The present invention relates to a novel Bacillus pumilus bilirubin oxidase, to the method for preparing same and also to the use thereof in particular for assaying bilirubin and for using enzymatic biofuel cells.

Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.

Abstract: The present invention relates to novel mutants of PQQ s-GDH containing an amino acid substitution in position 428 of the protein sequence of the wild type PQQ s-GDH of Acinetobacter calcoaceticus (SEQ.ID. NO:2). The invention also relates to the use of said PQQ s-GDH mutants for the development of glucose electrodes of interest in the assay of glucose, in particular of blood glucose in diabetic subjects, and for implementing biofuel cells that utilize glucose as fuel.