Patents by Inventor Nicolas Thevenin
Nicolas Thevenin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8648146Abstract: Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.Type: GrantFiled: June 6, 2012Date of Patent: February 11, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 8496123Abstract: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.Type: GrantFiled: July 24, 2006Date of Patent: July 30, 2013Assignee: GE Healtcare Bio-Sciences ABInventors: Andreas Axen, Anders Hagvall, Anders Larsson, Nicolas Thevenin
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Publication number: 20120245301Abstract: Disclosed is a method for activating a solid support material with epoxy groups and for immobilising ligands thereon, utilising phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilising a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.Type: ApplicationFiled: June 6, 2012Publication date: September 27, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: GUNNAR GLAD, JEAN-LUC MALOISEL, NICOLAS THEVENIN
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Patent number: 8217118Abstract: Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.Type: GrantFiled: November 22, 2006Date of Patent: July 10, 2012Assignee: GE Healthcare Bio-Sciences ABInventors: Gunnar Glad, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 7750129Abstract: The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.Type: GrantFiled: February 25, 2005Date of Patent: July 6, 2010Assignee: GE Healthcare Bio-Sciences ABInventors: Bo-Lennart Johansson, Hans J. Johansson, Anna Grönberg, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 7510657Abstract: The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatize the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatized scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.Type: GrantFiled: March 5, 2004Date of Patent: March 31, 2009Assignee: GE Healthcare Bio-Sciences ABInventors: Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20090048456Abstract: Disclosed is a method for activating a solid support material with epoxy groups and for immobilising ligands thereon, utilising phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilising a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.Type: ApplicationFiled: November 22, 2006Publication date: February 19, 2009Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Gunnar Glan, Jean-Luc Maloisel, NIcolas Thevenin
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Publication number: 20080199959Abstract: The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion.Type: ApplicationFiled: June 19, 2006Publication date: August 21, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Mattias Algotsson, Gunnar Glad, Nicolas Thevenin
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Publication number: 20080179248Abstract: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.Type: ApplicationFiled: July 24, 2006Publication date: July 31, 2008Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Andreas Axen, Anders Hagvall, Anders Larsson, Nicolas Thevenin
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Patent number: 7385040Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.Type: GrantFiled: February 24, 2005Date of Patent: June 10, 2008Assignee: GE Healthcare Bio-Sciences ABInventors: Bo-Lennart Johansson, Hans J. Johansson, Anders Ljunglöf, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 7320755Abstract: The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.Type: GrantFiled: March 5, 2004Date of Patent: January 22, 2008Assignee: GE Healthcare Bio-Sciences ABInventors: Bo-Lennart Johansson, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 7320754Abstract: The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.Type: GrantFiled: March 5, 2004Date of Patent: January 22, 2008Assignee: GE Healthcare Bio-Sciences ABInventors: Ulrika Carlsson, Jean-Luc Maloisel, Nicolas Thevenin
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Patent number: 7294743Abstract: The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase.Type: GrantFiled: October 18, 2004Date of Patent: November 13, 2007Assignee: GE Healthcare Bio-Sciences ABInventors: Mattias Algotsson, Philippe Busson, Nicolas Thevenin
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Publication number: 20070238088Abstract: Disclosed are hydrophilic functionalized silica compositions that are stable and do not show significant pH increases upon heat sterilization. Also provided are methods to make hydrophilic functionalized silica compositions by reacting acidic silica particles with hydrophilic organosilanes. Further provided are methods of separating components in a mixture using hydrophilic functionalized silica compositions.Type: ApplicationFiled: March 29, 2006Publication date: October 11, 2007Inventors: Slawomir Rubinsztajn, David Demoulpied, Omayra de Jesus, Gregory Goddard, James Van Alstine, Jan Larsson, Mohan Amaratunga, Nicolas Thevenin
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Publication number: 20070167613Abstract: The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.Type: ApplicationFiled: February 25, 2005Publication date: July 19, 2007Inventors: Bo-Lennart Johansson, Hans Johansson, Anna Gronberg, Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20070112178Abstract: The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.Type: ApplicationFiled: February 24, 2005Publication date: May 17, 2007Inventors: Bo-Lennart Johansson, Hans Johansson, Anders Ljunglof, Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20070106090Abstract: The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase. In a preferred embodiment the acryl amide derivative is an immobiline, preferably acrylamido agmatine. The preferred use of the immobilines is for production of 2D gels.Type: ApplicationFiled: October 18, 2004Publication date: May 10, 2007Inventors: Mattias Algotsson, Philippe Busson, Nicolas Thevenin
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Publication number: 20060188931Abstract: The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatise the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatised scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.Type: ApplicationFiled: March 5, 2004Publication date: August 24, 2006Inventors: Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20060175258Abstract: The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.Type: ApplicationFiled: March 5, 2004Publication date: August 10, 2006Inventors: Bo-Lennart Johansson, Jean-Luc Maloisel, Nicolas Thevenin
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Publication number: 20060175237Abstract: The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.Type: ApplicationFiled: March 5, 2004Publication date: August 10, 2006Inventors: Ulrika Carlsson, Jean-Luc Maloisel, Nicolas Thevenin