Abstract: A method for evaluating a test article provided by a subject for an attribute, such as a medical, industrial, veterinary, agricultural, food, or other attribute, is provided. The design includes providing a cassette comprising at least one ligand selected to match the attribute, applying the test article provided by the patient to the at least one ligand of the cassette, transmitting light energy toward the test article applied to the at least one ligand, sensing optical attributes of light energy provided from the test article applied to the at least one ligand, and providing sensed attributes of the light energy sensed to an electronic device.