Patents by Inventor Niels Klitgord

Niels Klitgord has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200270677
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: March 30, 2020
    Publication date: August 27, 2020
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: John F. REGAN, Dianna MAAR, Dawne N. SHELTON, Tina C. LEGLER, Samantha COOPER, Wei YANG, Eli A. HEFNER, Niels KLITGORD
  • Publication number: 20200234793
    Abstract: Provided are methods and systems for metagenomic analysis. Methods involve generating libraries and databases of taxonomic signatures informative of taxonomic information in a heterogeneous microbial sample. Methods also include identification of microorganisms and biochemical activities in the sample, including identification at a plurality of taxonomic levels.
    Type: Application
    Filed: March 1, 2018
    Publication date: July 23, 2020
    Inventors: Momchilo VUYISICH, Jason GANS, Niels KLITGORD, Po-E LI, Patrick CHAIN
  • Patent number: 10604789
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: March 31, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20190127781
    Abstract: A method to detect liver fibrosis or for the differential diagnosis of non-alchohlic fatty liver disease (NAFLD) in a subject is provided. The method comprises analyzing a biological sample from a subject to determine an intestinal microbiome signature for the subject, and inspecting the intestinal microbiome signature relative to a reference intestinal microbiome signature to detect presence or absence of liver fibrosis.
    Type: Application
    Filed: April 20, 2017
    Publication date: May 2, 2019
    Inventors: Shibu YOOSEPH, Rohit LOOMBA, Karen E. NELSON, Victor SEGURITAN, Weizhong LI, Tao LONG, Niels KLITGORD
  • Patent number: 9856525
    Abstract: System, including methods, apparatus, and compositions, for performing a digital assay with associated targets. In some embodiments, the assay of associated targets in partitions may lower the limit of detection (LOD) or otherwise increase assay sensitivity, accuracy, and/or specificity. The targets may represent spaced or overlapping regions of the same template and/or each may represent a region from a different complementary strand of the same template. In some embodiments, the associated targets may be provided by a type of biological particle, and the target content of partitions may be used to identify and quantify the type of biological particle in a sample.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: January 2, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Adam M. McCoy, Niels Klitgord
  • Publication number: 20170166957
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: January 20, 2017
    Publication date: June 15, 2017
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20170132357
    Abstract: Described are platforms, systems, media, and methods for providing a biologic information visual synthesis application, the biologic information including one or more of: genome data, microbiome data, and metabolome data.
    Type: Application
    Filed: November 10, 2016
    Publication date: May 11, 2017
    Inventors: Suzanne Brewerton, Bryan Coon, Chao Xie, Rafael Zuniga, Jhalley de Castro, Shibu Yooseph, Weizhong Li, Ryan Ulaszek, Niels Klitgord, Zhenxuan Yeo, Fabi Mulawadi, Aaron Friedman, Stephen Terrell, Adrianto Wirawan, Korkut Gule, Erhan Saygi, Andreas Hadimulyono, Yong Heng Tan, Thomas Sisk, Alexey Volochenko, Sean Blair, Aik Meng Ang, Kian Yong Lim, Daniel Zhang, Dmitry Bezyazychnyy, Qiang Wang, Xiaohui Liu, Ream Lim, Nikita Veshkurtsev, Marie Wong, Jason Piper, Miao Sun, Matthew Cloney, Bao Pham, Yaron Turpaz
  • Patent number: 9556475
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Grant
    Filed: August 12, 2014
    Date of Patent: January 31, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20150148250
    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
    Type: Application
    Filed: August 12, 2014
    Publication date: May 28, 2015
    Inventors: John F. Regan, Dianna Maar, Dawne N. Shelton, Tina C. Legler, Samantha Cooper, Wei Yang, Eli A. Hefner, Niels Klitgord
  • Publication number: 20140274786
    Abstract: System, including methods, apparatus, and compositions, for performing a digital assay with associated targets. In some embodiments, the assay of associated targets in partitions may lower the limit of detection (LOD) or otherwise increase assay sensitivity, accuracy, and/or specificity. The targets may represent spaced or overlapping regions of the same template and/or each may represent a region from a different complementary strand of the same template. In some embodiments, the associated targets may be provided by a type of biological particle, and the target content of partitions may be used to identify and quantify the type of biological particle in a sample.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventors: Adam M. McCoy, Niels Klitgord
  • Publication number: 20140162266
    Abstract: This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.
    Type: Application
    Filed: December 5, 2013
    Publication date: June 12, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Niels Klitgord, John Regan, Svilen Tzonev, Eli Hefner, Jennifer R. Berman