Abstract: The invention relates to a spot test method using a cupric chloride solution for detecting analytes in a sample which gives an immediate and distinctly colored reaction characteristic of the analyte. The invention also relates to a kit comprising the cupric chloride reagent.

Abstract: The invention relates to an assay for one or more analytes which comprises contacting a sample suspected of containing one or more analytes with a solid phase containing one or more different antigen specific antibodies separately immobilized to defined areas on the solid phase, followed by indirectly detecting the presence of bound antigen by titrating the unbound immobilized antibodies with a titrating antibody which is specific for the first antibody. This assay allows the simultaneous detection of a multiplicity of antigens in a single assay.

Abstract: The invention relates to a quantitative assay for an analyte which comprises contacting a sample containing an analyte with a solid phase support to immobilize said analyte, following by indirectly quantitating the presence of bound analyte by titrating the unoccupied binding sites with a titrating protein which is detectably labeled.