Patents by Inventor NIKHIL GHADE

NIKHIL GHADE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20160159853
    Abstract: A machine or apparatus for refolding a protein of interest produced recombinantly in a host cell in form of inclusion bodies is provided. The apparatus includes an inclusion bodies (IB) solubilisation tank to solubilise the inclusion bodies; a plurality of refolding vessels or reactors arranged in series to receive a refolding feed; a first tank connected to the IB solubilisation tank to hold diafiltration buffer or agent; a first diafiltration cartridge having at least one permeate end and one retentate end, connected to the IB solubilisation tank, through a first retentate end to feed the refolding feed to the IB solubilisation tank; a second tank, connected to the first permeate end of the diafiltration cartridge to receive and recycle the refolding buffer and each of the refolding vessels, for supplying refolding buffer or agent to each of the refolding vessels.
    Type: Application
    Filed: July 18, 2014
    Publication date: June 9, 2016
    Inventors: Sanjay SONAR, Archana KRISHNAN, Nikhil GHADE, Faiza SHAIKH
  • Publication number: 20160039868
    Abstract: A process for obtaining a refolded recombinant protein from an unfolded recombinant protein present in inclusion bodies isolated from wet cells harvested from a cell culture is provided. The process includes a) reducing the inclusion bodies by treating the inclusion bodies with a reducing buffer, to obtain reduced inclusion bodies; b) obtaining stable first intermediate state I1 of the unfolded recombinant protein present in the reduced inclusion bodies; and c) refolding the stable first intermediate state I1 of the unfolded recombinant protein, present in the reduced inclusion bodies, in refolding buffer to obtain a second intermediate state I2 that folds to produce the refolded recombinant protein.
    Type: Application
    Filed: March 21, 2014
    Publication date: February 11, 2016
    Applicant: BioGenomics Limited
    Inventors: Archana KRISHNAN, Sanjay SONAR, NIKHIL GHADE, FAIZA SHAIKH
  • Publication number: 20150376228
    Abstract: A process of refolding a recombinant protein from inclusion bodies (IBs) formed inside a host cell, is provided. The process includes homogenising a wet cells slurry to obtain a cell lysate; incubating the cell lysate with a reducing agent to obtain a reduced cell lysate; isolating reduced IBs from the reduced cell lysate to obtain isolated IBs; solubilising the isolated IBs with a denaturing agent to obtain solubilised IBs; and subjecting the solubilised IBs to a unit process of refolding to obtain a refolded recombinant protein.
    Type: Application
    Filed: February 21, 2014
    Publication date: December 31, 2015
    Applicant: BioGenomics Limited
    Inventors: Archana KRISHNAN, Sanjay SONAR, NIKHIL GHADE, FAIZA SHAIKH