Patents by Inventor Nikhil Phadke

Nikhil Phadke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10443088
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: October 15, 2019
    Assignee: HandyLab, Inc.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Publication number: 20190284606
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: February 22, 2019
    Publication date: September 19, 2019
    Inventors: Betty Wu, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
  • Patent number: 10364456
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: July 30, 2019
    Assignee: HANDYLAB, INC.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Publication number: 20150328638
    Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
    Type: Application
    Filed: May 22, 2015
    Publication date: November 19, 2015
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
  • Patent number: 9040288
    Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: May 26, 2015
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
  • Publication number: 20150118684
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 30, 2015
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 8852862
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: November 16, 2005
    Date of Patent: October 7, 2014
    Assignee: HandyLab, Inc.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Publication number: 20140030798
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: June 24, 2013
    Publication date: January 30, 2014
    Applicant: HANDYLAB, INC.
    Inventors: Betty WU, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
  • Patent number: 8470586
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: May 3, 2005
    Date of Patent: June 25, 2013
    Assignee: Handylab, Inc.
    Inventors: Betty Wu, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
  • Publication number: 20080262213
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: May 3, 2005
    Publication date: October 23, 2008
    Inventors: Betty Wu, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
  • Publication number: 20070292941
    Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
    Type: Application
    Filed: March 26, 2007
    Publication date: December 20, 2007
    Applicant: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
  • Publication number: 20070259348
    Abstract: Lyophilized pellets, suitable for use in a microfluidic device, and a method for preparing the same are described. The lyophilized pellets contain various biological reagents, or microparticles, and a cryoprotectant. The lyophilized pellets have a high degree of sphericity and are in the size range 0.5 to 35 ?L. The pellets are prepared by dispensing drops of reagent solution on to a cryogenically cooled plate, followed by subjecting to a vacuum.
    Type: Application
    Filed: May 3, 2006
    Publication date: November 8, 2007
    Applicant: HandyLab, Inc.
    Inventors: Nikhil Phadke, Michelle Manente, Kalyan Handique
  • Publication number: 20070184547
    Abstract: Methods and systems for preparing polynucleotide samples are disclosed. The invention includes a microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the polynucleotides, comprising: a cartridge receiving element, an insertable and removable cartridge, a heating element configured to heat one or more regions of the cartridge, and control circuitry, wherein the insertable cartridge comprises: a microfluidic component that is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analyzing the one or more polynucleotides. The invention further comprises a multi-sample cartridge for converting a number of samples, each containing one or more polynucleotides, into respective forms suitable for analyzing the polynucleotides, comprising: at least a first microfluidic component and a second microfluidic component.
    Type: Application
    Filed: October 11, 2006
    Publication date: August 9, 2007
    Inventors: Kalyan Handique, Jeff Williams, Sundaresh Brahmasandra, Nikhil Phadke, Betty Wu
  • Publication number: 20060166233
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: November 16, 2005
    Publication date: July 27, 2006
    Inventors: Betty Wu, John Althaus, Nikhil Phadke, Sundaresh Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer