Patent number: 11549119
Abstract: The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.
Type:
Grant
Filed:
April 6, 2017
Date of Patent:
January 10, 2023
Assignee:
ZUMUTOR BIOLOGICS, INC.
Inventors:
Sohang Chatterjee, Kavitha Iyer Rodrigues, Maloy Ghosh, Sunit Maity, Divya Unnikrishnan, Yogendra Manjunath Bangalore Muniraju, Sathyabalan Murugesan, Pavithra Mukunda, Bhargav Prasad, Veeresha Kamanagowda, Sanghamitra Bhattacharjee, Pravin Kumar Dakshinamurthy, Vivek Halan, Sankaranarayanan Srinivasan, Anuradha Hora, Bairavabalakumar Natarajan, Karthika Nair, Aswini Thanigaivel, Amol Maliwalave, Bharath Ravindra Shenoy, Sahana Bhima Rao, Subhra Prakash Chakrabarty, Ashvini Kumar Dubey, Amir Khan, Ankurina Sharma, Rashmi Sharma, Anurag Tiwari, Santosh Kumar, Shivani Patel, Nikitha M
Patent number: 11384354
Abstract: The present disclosure relates to a method of generating an antibody library, not limiting to a human naïve antibody gene expression library encompassing a pool of nucleic acid sequences derived from a natural antibody repertoire comprising humoral immunity from healthy and genetically diverse human populations. More specifically, the method employs a combination of phage and/or yeast antibody surface display concept which allows to screen large antibody library size and facilitates better folding of antibody structure. The present disclosure also relates to a human naïve antibody library generated by employing the process of the present disclosure, a set of primers employed in the method and application(s) of said antibody library.
Type:
Grant
Filed:
December 21, 2015
Date of Patent:
July 12, 2022
Assignee:
ZUMUTOR BIOLOGICS INC.
Inventors:
Sohang Chatterjee, Kavitha Iyer Rodrigues, Maloy Ghosh, Sunit Maity, Divya Unnikrishnan, Yogendra Manjunath Bangalore Muniraju, Sathyabalan Murugesan, Pavithra Mukunda, Bhargav Prasad, Veeresha Kamanagowda, Sanghamitra Bhattacharjee, Pravin Kumar Dakshinamurthy, Vivek Halan, Sankaranarayanan Srinivasan, Anuradha Hora, Bairavabalakumar Natarajan, Karthika Nair, Aswini Thanigaivel, Amol Maliwalave, Bharath Ravindra Shenoy, Sahana Bhima Rao, Subhra Prakash Chakrabarty, Ashvini Kumar Dubey, Amir Khan, Anurag Tiwari, Santosh Kumar, Shivani Patel, Nikitha M
Publication number: 20200283757
Abstract: The present disclosure relates to a method of generating an antibody library, not limiting to a human naïve antibody gene expression library encompassing a pool of nucleic acid sequences derived from a natural antibody repertoire comprising humoral immunity from healthy and genetically diverse human populations. More specifically, the method employs a combination of phage and/or yeast antibody surface display concept which allows to screen large antibody library size and facilitates better folding of antibody structure. The present disclosure also relates to a human naïve antibody library generated by employing the process of the present disclosure, a set of primers employed in the method and application(s) of said antibody library.
Type:
Application
Filed:
December 21, 2015
Publication date:
September 10, 2020
Inventors:
Sohang CHATTERJEE, Kavitha Iyer RODRIGUES, Maloy GHOSH, Sunit MAITY, Divya UNNIKRISHNAN, Yogendra Manjunath BANGALORE MUNIRAJU, Sathyabalan MURUGESAN, Pavithra MUKUNDA, Bhargav PRASAD, Veeresha KAMANAGOWDA, Sanghamitra BHATTACHARJEE, Pravin Kumar DAKSHINAMURTHY, Vivek HALAN, Sankaranarayanan SRINIVASAN, Anuradha HORA, Bairavabalakumar NATARAJAN, Karthika NAIR, Aswini THANIGAIVEL, Amol MALIWALAVE, Bharath Ravindra SHENOY, Sahana Bhima RAO, Subhra Prakash CHAKRABARTY, Ashvini Kumar DUBEY, Amir KHAN, Anurag TIWARI, Santosh KUMAR, Shivani PATEL, Nikitha M
Publication number: 20190119691
Abstract: The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.
Type:
Application
Filed:
April 6, 2017
Publication date:
April 25, 2019
Inventors:
Sohang CHATTERJEE, Kavitha Iyer RODRIGUES, Maloy GHOSH, Sunit MAITY, Divya UNNIKRISHNAN, Yogendra Manjunath Bangalore MUNIRAJU, Sathyabalan MURUGESAN, Pavithra MUKUNDA, Bhargav PRASAD, Veeresha KAMANAGOWDA, Sanghamitra BHATTACHARJEE, Pravin Kumar DAKSHINAMURTHY, Vivek HALAN, Sankaranarayanan SRINIVASAN, Anuradha HORA, Bairavabalakumar NATARAJAN, Karthika NAIR, Aswini THANIGAIVEL, Amol MALIWALAVE, Bharath Ravindra SHENOY, Sahana Bhima RAO, Subhra Prakash CHAKRABARTY, Ashvini Kumar DUBEY, Amir KHAN, Ankurina SHARMA, Rashmi SHARMA, Anurag TIWARI, Santosh KUMAR, Shivani PATEL, Nikitha M