Abstract: A method of removal and recovery of hexavalent chromium from effluents by passive-active biological process is described. The method may include adsorption of the hexavalent chromium by passive biological process of removal of hexavalent chromium from effluents by adsorbing the hexavalent chromium using a treated and conditioned Limonia acidissima biomass particles. The method may further include conducting bioreduction of the leftover chromium present in the effluent in low concentration by using active bacterial culture for complete removal of hexavalent chromium. The treated and conditioned Limonia acidissima biomass particles having the adsorbed hexavalent chromium may be burnt in a furnace at 400° C. to obtain an ash containing hexavalent chromium. The ash obtained may be mixed with water and filtered to obtain a hexavalent chromium solution which could be recycled and reused.