Abstract: The present invention relates to a computer implemented method for controlling a fluid coupling network (170), the fluid coupling network (170) being configured to be fluidly couplable to bioreactor (110), a gas source (120), a buffer source (140), a waste port (150), a processing system (160) and a conduit reservoir (208), the fluid coupling network (170) comprising a barrier unit (220) configured to sterilely separate fluid paths within the fluid coupling network (170), the fluid coupling network (170) being controllable to sample the bioreactor (110), the method comprising obtaining (510) a fluid sample from the bioreactor (110), wherein the step of obtaining the fluid sample comprises controlling a flow of fluid from the bioreactor (110) via the conduit reservoir (208) and the fluid coupling network (170) to the waste port (150), to fill the conduit reservoir (208) with the fluid from the bioreactor (110), providing (530) the fluid sample, wherein the step of providing the fluid sample comprises to provid

Abstract: Disclosed is a chromatography system (100) comprising: plural modules (1-25) including at least one pump and a column valve unit (8) connectable to plural chromatography columns; and a controller (600), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit. The system includes a housing (110) into which the plural modules (1-25) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.

Abstract: Disclosed is a chromatography system (100) comprising: plural modules (1-25) including at least one pump and a column valve unit (8) connectable to plural chromatography columns; and a controller (600), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit. The system includes a housing (110) into which the plural modules (1-25) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.

Abstract: The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device configured to remove air from a fluid and comprising the nozzle.

Abstract: Disclosed is a method for determining an operating flow rate for a chromatographic column (4) in an HPLC system (1). The method comprises: measuring/calculating a pressure of the system (1) without the chromatographic column (4) for one or more flow rates; fitting a function to the flow rate(s) and corresponding pressure(s), calculating from the function and a predetermined recommended flow rate for the chromatographic column (4) a system pressure drop at the predetermined recommended flow rate. An operating flow rate is determined by summing the system pressure drop and a maximal column pressure limit, and determining a contribution of the system pressure drop to the summed pressure. If this contribution exceeds 1% an operating flow rate for the column is determined to a flow rate that corresponds to a pressure at a pressure monitor arranged before the column that is lower than the predetermined maximum column pressure limit.

Abstract: The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device 200 configured to remove air from a fluid and comprising the nozzle.

Abstract: The present invention relates to a method for determining size of biomolecules separated in a medium by an electric field using marker molecules of known size, comprising —(101) detecting a plurality of bands and forming a detected marker sequence and a detected unknown sequence based on a separation criterion, —(102) determining band properties for each detected band, —(103) comparing the band properties of the detected bands of the detected marker sequence with known band properties for a plurality of marker molecules forming a known marker sequence and assigning a score to each comparison, said score being based on at least one of relative distance, relative intensity, expected distance and expected intensity between bands, —(104) selecting the comparison with the highest score and associating all or a subset of the detected bands of the detected marker sequence with said plurality of marker molecules of the known marker sequence in accordance with said comparison to determine size of the all or a subset o

Abstract: A method for fraction collection and a fraction collection system (100) comprising: —a dispensing device (23) including an outlet (24) configured for dispensing fractions of a liquid received from a connected system; —plural receptacles (25) configured for receiving one or more of said fractions, wherein the receptacles (25) and the dispensing device (23) are movable in relation to each other into a number of different positions such that the dispensing device (23) is capable of dispensing a respective fraction of said liquid into one or more of the receptacles (25); —a waste collection device (27), which is arranged in the fraction collection system (100) such that it is movable into at least a first and a second position wherein, in said first position, an inlet (29) of the waste collection device (27) is positioned below the outlet (24) of the dispensing device (23) such that liquid being dispensed from the outlet (24) of the dispensing device (23) is received in the waste collection device (27) and wherei

Abstract: The present invention relates to a nozzle for an air trapping device configured to remove air from a fluid, the nozzle comprising a body having an input opening configured to receive the fluid and an output opening configured to distribute the fluid along an edge of the output opening, wherein the edge comprises a control element configured to reduce surface tension of the fluid. The present invention further relates to an air trapping device 200 configured to remove air from a fluid and comprising the nozzle.

Abstract: Disclosed is a chromatography system (100) comprising: plural modules (1-25) including at least one pump and a column valve unit (8) connectable to plural chromatography columns; and a controller (600), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit. The system includes a housing (110) into which the plural modules (1-25) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.

Abstract: The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base.

Abstract: The present invention relates to a method for determining size of biomolecules separated in a medium by an electric field using marker molecules of known size, comprising—(101) detecting a plurality of bands and forming a detected marker sequence and a detected unknown sequence based on a separation criterion, —(102) determining band properties for each detected band, —(103) comparing the band properties of the detected bands of the detected marker sequence with known band properties for a plurality of marker molecules forming a known marker sequence and assigning a score to each comparison, said score being based on at least one of relative distance, relative intensity, expected distance and expected intensity between bands, —(104) selecting the comparison with the highest score and associating all or a subset of the detected bands of the detected marker sequence with said plurality of marker molecules of the known marker sequence in accordance with said comparison to determine size of the all or a subset of

Abstract: The invention discloses a method for correcting a baseline in a chromatogram obtained using a buffer with at least one UV-absorbing component which is a weak acid or a weak base.

Abstract: A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

Abstract: A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

Abstract: A method of preparing a liquid mixture of controlled pH including (i) one or more buffering species, (ii) an acid or alternatively a base, (iii) optionally a salt; and (iv) a solvent wherein the proportions of the different components ((I) to (iv)) of the mixture are concomitantly varied in such a way as to take into account of the interrelationship of the ionic strength and the pH of the mixture.