Abstract: Needleless connector disinfecting devices are provided that include a body having a top surface and a bottom surface, a cavity in the body, the cavity configured to receive a needleless connector, an activation arm disposed on the body, a cartridge housing disposed on the activation arm, a swab cartridge disposed in the cartridge housing, and a disposal chamber disposed in the body, the disposal chamber configured to receive used cleaning swabs. The activation arm is configured to slidably move the cartridge housing on the top surface of the body between a non-biased position in which a bottommost cleaning swab in the swab cartridge is in contact with an end portion of a needleless connector and a biased position in which the cartridge housing is disposed over the disposal chamber. Additional needleless connector disinfecting devices and methods of using needleless connector disinfecting devices are also provided.