Abstract: A process for chemical crosslinking of tissue joining partners including crosslinkable groups, such as free amino groups, by a suitable crosslinking agent under static, quasi-static or periodic pulsatile pressure compression. The compression can be in a defined overlap region for seamless, dense and tight tissue closure. This results in a seamless, homogeneous, and at the same time mechanically stable connection/joining of tissue/tissue components. Seamless connected tissue is provided that includes a piece of tissue having at least two tissue parts overlapping each other and the at least two tissue parts overlapping each other that are materially bonded to each other via crosslinked groups of the tissue.

Abstract: A method for chemically crosslinking and optionally shaping (including thickness reduction) substantially non-cross-linked tissue, in particular biological tissue, using a permeable material layer. The method can be used in combination with a stepless pressure load/compression on the tissue, so as to always allow fluid transport into and out of the tissue to be treated under the chemical cross-linking; along the lines of drainage. The method can be carried out under different pressure loads/compressions, in particular in order to specifically influence the resulting tissue properties, and, for example, to bring about a homogeneous thickness distribution of the tissue and/or to deliberately and specifically place inhomogeneities, if desired. Furthermore, the method allows the introduction/imprinting of a consistent three-dimensional shape into the tissue to be treated, e.g. of inhomogeneities in the thickness of the tissue.

Abstract: A process for three-dimensional shaping of a biological and/or artificial tissue/tissue component. The process is consistent for shaping tissues and can produce a desired three-dimensional and permanent shaping of the tissue/tissue component via crosslinking. A suitable crosslinking agent is used in combination with shaped body and a granulate.

Abstract: A method for preparing tissue, in particular pericardial tissue, in particular for a heart valve prosthesis, the method including: decellularizing the tissue; subjecting the decellularized tissue to a cross-linking solution including glutaraldehyde; subjecting the tissue to a shape- and structure-stabilizing step, in which the tissue is exposed to a first solution containing glycerol and is exposed to a second solution containing polyethylene glycol; and drying the tissue after the shape- and structure-stabilizing step.

Abstract: A method of preparing biological tissue for use as a component of an implant, in particular as part of a vascular implant, more particularly as part of a heart valve prosthesis, which can be implanted by a catheter. The biological tissue is decellularized using a detergent, which includes surfactin and deoxycholic acid (DCA).

Abstract: A method for preparing tissue, in particular pericardial tissue, in particular for a heart valve prosthesis, the method including: decellularizing the tissue; subjecting the decellularized tissue to a cross-linking solution including glutaraldehyde; subjecting the tissue to a shape- and structure-stabilizing step, in which the tissue is exposed to a first solution containing glycerol and is exposed to a second solution containing polyethylene glycol; and drying the tissue after the shape- and structure-stabilizing step.