Abstract: The invention relates to a method and a device for the highly sensitive parallel detection and quantitative determination of analytes in liquid samples. According to said method, total internal reflection fluorescence (TIRF) is used in combination with a binding inhibition test on a specially coated support. The inventive method makes it possible to quickly analyze different types of liquids, such as drinking water, fruit juices, milk, serum, blood plasma, urine, etc., while allowing samples to be analyzed simultaneously regarding several different analytes, including hormones, antibiotics, pesticides, pharmaceuticals, drugs, and other molecules or molecular complexes, for example.