Abstract: The present disclosure provides a self-powered, portable, and high-throughput Surface-Enhanced Raman Spectroscopy (SERS) biosensing platform, including: a handheld Raman spectrometer, a friction nanogenerator, a superhydrophobic metal substrate, and a high-voltage electrode array. The friction nanogenerator has one end connected to the superhydrophobic metal substrate, and the other end connected to the high-voltage electrode array. The high-voltage electrode array is disposed above the superhydrophobic metal substrate. The friction nanogenerator is used for providing an alternating voltage to the high-voltage electrode array. The high-voltage electrode array is used for oscillating droplets on the superhydrophobic metal substrate according to the alternating voltage, and generating an eddy current to heat the droplets and accelerate evaporation of the droplets. The handheld Raman spectrometer is used for detecting the enriched droplets, to obtain a SERS spectrum of an analyte.