Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. The thermoelectric devices are operably coupled to the sample block, wherein each thermoelectric device includes a housing for a thermal sensor and a thermal control interface with a controller. Each thermoelectric device is further configured to operate independently from each other to provide a substantially uniform temperature profile throughout the sample block.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. The thermoelectric devices are operably coupled to the sample block, wherein each thermoelectric device includes a housing for a thermal sensor and a thermal control interface with a controller. Each thermoelectric device is further configured to operate independently from each other to provide a substantially uniform temperature profile throughout the sample block.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: The disclosure provides SLE biomarkers. The disclosure further provides kits and methods of diagnosing, prognosing, and stratifying subjects with the disease by utilizing SLE biomarkers.

Abstract: A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. The thermoelectric devices are operably coupled to the sample block, wherein each thermoelectric device includes a housing for a thermal sensor and a thermal control interface with a controller. Each thermoelectric device is further configured to operate independently from each other to provide a substantially uniform temperature profile throughout the sample block.

Abstract: The present invention provides reagents and methods for breast cancer detection.

Abstract: The disclosure features methods that include: providing a substrate that includes (i) a nucleic acid (e.g., DNA or RNA) encoding a hybrid amino acid sequence including a test amino acid sequence and an affinity tag, and (ii) a binding agent that recognizes the affinity tag; contacting the substrate with a translation effector to thereby translate the hybrid amino acid sequence; maintaining the substrate under conditions permissive for the hybrid amino acid sequence to bind the binding agent; and removing the nucleic acid from the substrate. In one embodiment, the substrate includes a plurality of positionally-distinguishable addresses, for example, each includes a different nucleic acid. The addresses can be located a regularly or irregularly spaced locations.

Abstract: The disclosure features methods that include: providing a substrate that includes (i) a nucleic acid (e.g., DNA or RNA) encoding a hybrid amino acid sequence including a test amino acid sequence and an affinity tag, and (ii) a binding agent that recognizes the affinity tag; contacting the substrate with a translation effector to thereby translate the hybrid amino acid sequence; maintaining the substrate under conditions permissive for the hybrid amino acid sequence to bind the binding agent; and removing the nucleic acid from the substrate. In one embodiment, the substrate includes a plurality of positionally-distinguishable addresses, for example, each include a different nucleic acid. The addresses can be located a regularly or irregularly spaced locations.

Abstract: The disclosure features methods that include: providing a substrate that includes (i) a nucleic acid (e.g., DNA or RNA) encoding a hybrid amino acid sequence including a test amino acid sequence and an affinity tag, and (ii) a binding agent that recognizes the affinity tag; contacting the substrate with a translation effector to thereby translate the hybrid amino acid sequence; maintaining the substrate under conditions permissive for the hybrid amino acid sequence to bind the binding agent; and removing the nucleic acid from the substrate. In one embodiment, the substrate includes a plurality of positionally-distinguishable addresses, for example, each include a different nucleic acid. The addresses can be located a regularly or irregularly spaced locations.

Abstract: Arrays of polypeptides can be generated by translation of nucleic acid sequences encoding the polypeptides at individual addresses on the array. This allows for the rapid and versatile development of a polypeptide microarray platform for analyzing and manipulating biological information. In one embodiment, one or more nucleic acids that include a coding region and an anchoring agent are to stably attached to the substrate. The substrate can also be modified to include a binding agent.