Abstract: A waste collection cart assembly for collecting waste material from a suction line during a medical procedure. The waste collection cart assembly includes a housing having a top portion defining a work surface. A waste container is coupled to the housing for collecting the waste material from the suction line. A manifold receiver defining an opening is coupled to the waste container. A manifold having a specimen trap is received within the opening of the manifold receiver. A vacuum source is coupled to the waste container. The vacuum source provides a vacuum on the waste container to draw the waste material from the suction line through the manifold and into the waste container. The specimen trap collects a specimen from the waste material while being drawn into the waste container. The specimen may be transferred to a specimen container disposed on the work surface.