Patents by Inventor Nobuhiko Yamashita

Nobuhiko Yamashita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Anti-PAH monoclonal antibodies and cell lines producing the same
    Patent number: 6277964
    Abstract: The present invention provides monoclonal antibodies suitable for monitoring polycyclic aromatic hydrocarbons (PAHs) by an immunoasay, hybridoma cell lines producing said antibodies, and an immunoassay for analyzing PAHs in a sample using said monoclonal antibodies. In addition, the present invention provides PAH conjugates useful as an immunogen in preparing said antibodies and as a standard substance in a competitive assay.
    Type: Grant
    Filed: March 11, 1999
    Date of Patent: August 21, 2001
    Assignee: Osaka Gas Company Limited
    Inventors: Nobuhiko Yamashita, Kazunobu Miura, Naoya Ichimura, Chiwa Kataoka, Junko Sakaki, Yutaka Ohtani, Hirohisa Kitagawa
  • Anti-human calcitonin monoclonal antibodies and an immunoassay utilizing said antibodies
    Patent number: 6133427
    Abstract: Monoclonal antibodies having a high affinity for human calcitonin, particularly monoclonal antibodies suitable for a sandwich immunoassay are disclosed. Also disclosed are hybridomas producing said monoclonal antibodies and a sandwich immunoassay utilizing said antibodies for determining human calcitonin in blood.
    Type: Grant
    Filed: December 23, 1997
    Date of Patent: October 17, 2000
    Assignee: Osaka Gas Company Limited
    Inventors: Nobuhiko Yamashita, Manabu Nakamoto, Kazunobu Miura, Chiwa Kataoka, Junko Sakaki
  • Method for preparing peptides and proteins utilizing ribosome
    Patent number: 5556768
    Abstract: A novel method for synthesizing peptides and proteins having a desired amino acid sequence by utilizing an aminoacyl-tRNA and misaminoacyl-tRNA as well as an artificial mRNA in a system for in vitro protein synthesis is provided. According to the present method, desired peptides and proteins can be synthesized using naturally-occurring and non naturally-occurring amino acids. Accordingly, the present invention provides a fundamental technique which is important in the fields of various industries and various fundamental researches such as biochemistry, molecular biology and protein engineering.
    Type: Grant
    Filed: June 28, 1994
    Date of Patent: September 17, 1996
    Assignee: Osaka Gas Company Limited
    Inventor: Nobuhiko Yamashita
  • Method for preparing aminoacyl- and misaminoacyl-tRNA
    Patent number: 5439811
    Abstract: A method for preparing aminoacyl- and misaminoacyl-tRNAs utilizing a specific aminoacyl-tRNA hydrolase is provided. According to the present method, aminoacyl-tRNAs consisting of a tRNA and a corresponding amino acid, or misaminoacyl-tRNAs consisting of a tRNA and a non-corresponding amino acid or an amino acid derivative, can be prepared. In a specific embodiment, L-tyrosyl-tRNA.sup.Tyr is prepared using aminoacyl-tRNA hydrolase from Fusarium culmorum or Artemia salina. In another embodiment a misacylated tRNA, L-phenylalanyl-tRNA.sup.Tyr, is prepared using the aminoacyl-tRNA hydrolase.
    Type: Grant
    Filed: March 14, 1994
    Date of Patent: August 8, 1995
    Assignee: Osaka Gas Company Limited
    Inventors: Nobuhiko Yamashita, Junichi Tohyama, Chiwa Kataoka, Kazunobu Miura
  • Monoclonal antibody specifically recognizing N-terminal portion of human calcitonin
    Patent number: 5330909
    Abstract: A monoclonal antibody specifically recognizing N-terminal portion of human calcitonin (hCT), a hybridoma producing said monoclonal antibody, a process for preparing said monoclonal antibody, and a method for detecting hCT which comprises the use of said monoclonal antibody are provided.The above monoclonal antibody permits detection of hCT with high sensitivity.
    Type: Grant
    Filed: October 8, 1992
    Date of Patent: July 19, 1994
    Assignee: Osaka Gas Company Limited
    Inventors: Nobuhiko Yamashita, Manabu Nakamoto, Ryoichi Okura, Isao Matsushita