Patents by Inventor Nobuki Tada

Nobuki Tada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11702678
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Grant
    Filed: July 29, 2021
    Date of Patent: July 18, 2023
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Junji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
  • Patent number: 11702679
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Grant
    Filed: July 29, 2021
    Date of Patent: July 18, 2023
    Assignee: TOYOTA JIDOSHA KABISHIKI KAISHA
    Inventors: Junji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
  • Patent number: 11299757
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Grant
    Filed: November 5, 2018
    Date of Patent: April 12, 2022
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Junji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
  • Publication number: 20220073896
    Abstract: Provided are excellent L-arabinose metabolic genes that function in yeasts. Provided is an L-arabinose metabolic gene cluster including an L-arabinose isomerase gene specified by a predetermined SEQ ID, an L-ribulokinase gene specified by a predetermined SEQ ID, and an L-ribulose-5-phosphate-4-epimerase gene specified by a predetermined SEQ ID.
    Type: Application
    Filed: December 24, 2019
    Publication date: March 10, 2022
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Rie HIRAO, Nobuki TADA, Toru ONISHI
  • Publication number: 20210363552
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Application
    Filed: July 29, 2021
    Publication date: November 25, 2021
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Junji ITO, Toru ONISHI, Nobuki TADA, Rie HIRAO
  • Publication number: 20210363551
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Application
    Filed: July 29, 2021
    Publication date: November 25, 2021
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Junji ITO, Toru ONISHI, Nobuki TADA, Rie HIRAO
  • Publication number: 20210180092
    Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
    Type: Application
    Filed: November 5, 2018
    Publication date: June 17, 2021
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Junji ITO, Toru ONISHI, Nobuki TADA, Rie HIRAO
  • Publication number: 20200399659
    Abstract: A stable transformant, in which a gene of interest is incorporated into the genome, is simply and efficiently produced. A method for producing a transformant, comprising a step of introducing into a host, linear genome-introduced nucleic acid fragment(s) comprising a gene of interest and a helper plasmid for transformation having a pair of homologous recombination sequences for incorporation of the linear genome-introduced nucleic acid fragment(s), and then selecting a transformant, in which the gene of interest is incorporated into the predetermined position in the host genome and the gene of interest is expressed therein.
    Type: Application
    Filed: June 23, 2020
    Publication date: December 24, 2020
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Toru Onishi, Nobuki Tada
  • Patent number: 10745684
    Abstract: An acetic acid metabolizing ability of a recombinant yeast strain having xylose-metabolizing ability is to be improved. In such a recombinant yeast strain having xylose-metabolizing ability, the acetaldehyde dehydrogenase gene has been introduced and a gene encoding NADH dehydrogenase involved in reoxidation of cytoplasmic NADH on the mitochondrial outer membrane has been suppressed.
    Type: Grant
    Filed: August 1, 2018
    Date of Patent: August 18, 2020
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Nobuki Tada, Toru Onishi, Junji Ito, Rie Hirao
  • Patent number: 10533169
    Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.
    Type: Grant
    Filed: June 27, 2018
    Date of Patent: January 14, 2020
    Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Satoshi Katahira, Risa Nagura, Kenro Tokuhiro, Nobuhiro Ishida, Chie Imamura, Toru Onishi, Noriko Yasutani, Nobuki Tada
  • Publication number: 20190211366
    Abstract: The invention is intended to metabolize acetic acid in a medium at the time of culture, such as ethanol fermentation by yeast, and to reduce acetic acid concentration. Specifically, the invention relates to a recombinant yeast resulting from introduction of the acetaldehyde dehydrogenase gene (EC 1.2.1.10) and regulation of an enzyme involved with trehalose accumulation.
    Type: Application
    Filed: May 8, 2017
    Publication date: July 11, 2019
    Applicant: Toyota Jidosha Kabushiki Kaisha
    Inventors: Toru ONISHI, Nobuki TADA
  • Publication number: 20190106719
    Abstract: This invention is aimed at improving an ethanol fermentation ability of a recombinant yeast strain having an ability of assimilating pentose, such as xylose or arabinose. The recombinant yeast strain haying an ability of assimilating pentose is obtained by lowering activity of a gene involved in upstream of glyceraldehyde-3-phosphate in the Embden-Meyerhof pathway.
    Type: Application
    Filed: October 9, 2018
    Publication date: April 11, 2019
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Toru ONISHI, Nobuki TADA, Junji ITO, Rie HIRAO
  • Publication number: 20190040379
    Abstract: An acetic acid metabolizing ability of a recombinant yeast strain having xylose-metabolizing ability is to be improved. In such a recombinant yeast strain having xylose-metabolizing ability, the acetaldehyde dehydrogenase gene has been introduced and a gene encoding NADH dehydrogenase involved in reoxidation of cytoplasmic NADH on the mitochondrial outer membrane has been suppressed.
    Type: Application
    Filed: August 1, 2018
    Publication date: February 7, 2019
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Nobuki Tada, Toru Onishi, Junji Ito, Rie Hirao
  • Publication number: 20180298369
    Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.
    Type: Application
    Filed: June 27, 2018
    Publication date: October 18, 2018
    Applicants: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Satoshi KATAHIRA, Risa NAGURA, Kenro TOKUHIRO, Nobuhiro ISHIDA, Chie IMAMURA, Toru ONISHI, Noriko YASUTANI, Nobuki TADA
  • Patent number: 10036005
    Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: July 31, 2018
    Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Satoshi Katahira, Risa Nagura, Kenro Tokuhiro, Nobuhiro Ishida, Chie Imamura, Toru Onishi, Noriko Yasutani, Nobuki Tada
  • Patent number: 10017788
    Abstract: The invention is intended to improve xylose assimilation ability and ethanol fermentation ability in a xylose-assimilating yeast into which a xylose isomerase gene has been introduced. The amount of NADH produced by the recombinant yeast into which the xylose isomerase gene had been introduced as a result of the enzymatic reaction of acetohydroxy acid reductoisomerase is lowered.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: July 10, 2018
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Toru Onishi, Nobuki Tada, Satoshi Katahira, Risa Nagura, Nobuhiro Ishida
  • Publication number: 20160122784
    Abstract: The invention is intended to improve xylose assimilation ability and ethanol fermentation ability in a xylose-assimilating yeast into which a xylose isomerase gene has been introduced. The amount of NADH produced by the recombinant yeast into which the xylose isomerase gene had been introduced as a result of the enzymatic reaction of acetohydroxy acid reductoisomerase is lowered.
    Type: Application
    Filed: June 9, 2014
    Publication date: May 5, 2016
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Toru ONISHI, Nobuki TADA, Satoshi KATAHIRA, Risa NAGURA, Nobuhiro ISHIDA
  • Publication number: 20160040151
    Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.
    Type: Application
    Filed: March 28, 2014
    Publication date: February 11, 2016
    Inventors: Satoshi KATAHIRA, Risa NAGURA, Kenro TOKUHIRO, Nobuhiro ISHIDA, Chie IMAMURA, Toru ONISHI, Noriko YASUTANI, Nobuki TADA
  • Publication number: 20160002674
    Abstract: The invention is intended to metabolize acetic acid and to lower acetic acid concentration in a medium at the time of xylose assimilation and ethanol fermentation by a yeast strain having xylose-metabolizing ability. The method for producing ethanol comprises a step of culturing recombinant yeast strains resulting from introduction of a xylose isomerase gene and an acetaldehyde dehydrogenase gene into a medium containing xylose, so as to perform ethanol fermentation.
    Type: Application
    Filed: February 27, 2014
    Publication date: January 7, 2016
    Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Toru ONISHI, Nobuki TADA, Noriko YASUTANI, Satoshi KATAHIRA, Nobuhiro ISHIDA, Risa NAGURA
  • Patent number: 8741625
    Abstract: Thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which are the offensive odor components, are provided. Disclosed are thermophilic microorganisms having a deodorizing ability for short chain fatty acids, which belong to Bacillus licheniformis.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: June 3, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Nobuki Tada, Hibiki Matsushita, Toshiaki Kimura