Abstract: A stable pharmaceutical preparation containing a human growth hormone having a molecular weight of about 20,000, or a derivative thereof, can be formulated by adding a water soluble heterocyclic compound, such as creatinine, a salt of acetyltryptophane and nicotinamide, to prevent the insolubilization of the human growth hormone or the derivative thereof in an aqueous solution.

Abstract: A human growth hormone can be purified by allowing a solution containing the human growth hormone to contact with a blue pigment-bonded carrier in order to selectively adsorb the human growth hormone, then eluting the human growth hormone with an eluant of high ionic strength or an eluant containing a protein denaturing agent. This method readily enables the mass production of a highly purified human growth hormone.

Abstract: A method for the production of human tissue type plasminogen activator (human t-PA) using cells is disclosed. The method comprises producing the human t-PA in parallel with the growth of the cells or after the cells have been grown. To the cell culture bicarbonate ion is added at a concentration equivalent to 3 to 10 g/l of NaHCO.sub.3 based on the true volume of the medium to increase the osmotic pressure of the medium to a range of 350 to 520 milliosmoles/liter.

Abstract: A method for the production of human tissue type plasminogen activator (tPa) using cells is disclosed. The method includes a supplementation of a p-aminomethyl benzoic acid derivative to a cell culture medium or a tPA producing medium and further an increase of osmotic pressure in the medium to 350 milliosmoles or more/liter. The invention provides a method for producing single-chain tPA in a high concentration and with a relatively small amount of double-chain tPA in the medium.

Abstract: The invention relates to a procedure for chemically determining the base sequence of DNA and, in particular, its step of cleaving the labeled DNA having undergone a base-selective chemical modification at the position of the modified base. More particularly, it relates to a method for cleaving DNA which comprises modifying DNA base-selectively with chemical reagents, bringing the resulting reaction mixture into contact with an adsorbent to adsorb the chemically modified DNA on the adsorbent, washing the adsorbent with a washing fluid to remove any chemical modification reagents from the adsorbent, bringing the adsorbent into contact with an eluent to elute the chemically modified DNA adsorbed thereon, concentrating the resulting eluate and then heating it together with a cleavage reagent.