Abstract: Radionuclide can be easily removed from a solution under aerobic condition by culturing Euglena in contact with radionuclide in a solution that is suitable for viability Euglena to thus transfer said radionuclide to Euglena and separating said Euglena comprising radionuclide from said solution.

Abstract: The present invention provides a process for producing biologically active recombinant yeast protein disulfide isomerase, which includes the steps of: a) deleting, substituting, or adding one or more bases in a region encoding an endoplasmic reticulum retention signal in a gene encoding protein disulfide isomerase of yeast to modify the gene so as not to encode part or all of the endoplasmic reticulum retention signal; b) incorporating the modified gene into an expression vector; c) transforming host cells with the expression vector; and d) culturing the host cells transformed with the expression vector in a culture medium kept at a nearly neutral pH, thereby causing protein disulfide isomerase to be secreted in an active state outside the host cells. According to the process of the invention, recombinant yeast protein disulfide isomerase can be secreted in a large amount in a biologically active state into a culture medium, and can be collected by a simple purification method.

Abstract: A screw member having a plurality of heads by providing one or more thin neck portions to be sheared at a predetermined torque, in which a collar having a skirt portion with its one part being cut away is fitted on the thin neck portion from the lateral side to expose the outer head part alone and to engage the cap member fitted from the side of the bearing surface of the inner head part of the screw member, and the cap member is inserted between the skirt of the collar and the inner head part to thereby prevent the collar from slipping out.