Patents by Inventor Nobuyuki Kurosawa
Nobuyuki Kurosawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11970549Abstract: The invention provides an antibody that specifically binds to a 5? to 3? exonuclease domain of a DNA polymerase, or a fragment thereof. The antibody inhibits the 5? to 3? exonuclease activity of a DNA polymerase, or a fragment thereof.Type: GrantFiled: December 10, 2021Date of Patent: April 30, 2024Assignees: TOYOBO CO., LTD., NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Takafumi Yoshikane, Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20230140801Abstract: The invention provides an antibody that specifically binds to a 5? to 3? exonuclease domain of a DNA polymerase, or a fragment thereof. The antibody inhibits the 5? to 3? exonuclease activity of a DNA polymerase, or a fragment thereof.Type: ApplicationFiled: December 10, 2021Publication date: May 4, 2023Applicants: TOYOBO CO., LTD., NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Takafumi YOSHIKANE, Nobuyuki KUROSAWA, Masaharu ISOBE
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Patent number: 10416165Abstract: A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell.Type: GrantFiled: August 10, 2016Date of Patent: September 17, 2019Assignee: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20180292407Abstract: A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell.Type: ApplicationFiled: August 10, 2016Publication date: October 11, 2018Applicant: National University Corporation University of ToyamaInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Patent number: 9625464Abstract: A method which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker is provided. Further disclosed is a fluorescent probe wherein the staining selectivity for the endoplasmic reticulum of cells is higher than the staining selectivity for cell organelles other than the endoplasmic reticulum. Also disclosed is a method for identifying plasma cells and plasmablasts which includes staining cells derived from lymph node tissue or similar by using this probe, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells. Also disclosed is a fluorescent probe wherein the staining selectivity for cell nuclei is higher than the staining selectivity for cell organelles other than the cell nuclei.Type: GrantFiled: March 22, 2011Date of Patent: April 18, 2017Assignee: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Patent number: 9487583Abstract: [Problem] To provide a method that efficiently produces antigen-specific monoclonal antibodies from a wide range of animal species, and to provide a new antigen-specific monoclonal antibody using this technique. [Solution] A nonhuman animal is immunized with a target antigen, lymph fluid or the like is collected from the immunized nonhuman animal, or lymph fluid or the like is collected from a human having antibodies to the target antigen, the collected lymph fluid or the like is combined with (1) a labeled target antigen and (2) a marker that can selectively binds to plasma cells and/or plasmablasts, and cells that have bound to (1) the labeled target antigen and (2) the marker are then selected.Type: GrantFiled: March 28, 2012Date of Patent: November 8, 2016Assignee: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Patent number: 9348403Abstract: Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed. A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.Type: GrantFiled: March 31, 2015Date of Patent: May 24, 2016Assignee: Renesas Electronics CorporationInventors: Satoshi Yoshida, Nobuyuki Kurosawa, Kenta Sasaki
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Publication number: 20150268715Abstract: Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed. A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.Type: ApplicationFiled: March 31, 2015Publication date: September 24, 2015Inventors: Satoshi Yoshida, Nobuyuki Kurosawa, Kenta Sasaki
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Patent number: 9015508Abstract: Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed. A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.Type: GrantFiled: December 10, 2011Date of Patent: April 21, 2015Assignee: Renesas Electronics CorporationInventors: Satoshi Yoshida, Nobuyuki Kurosawa, Kenta Sasaki
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Patent number: 8999673Abstract: Provided is a method for selectively obtaining, for a given target gene, a “joined DNA fragment” wherein just a target gene fragment is joined with desired other DNA fragments, regardless of whether a DNA fragment containing a target gene sequence has been purified. In the provided method, a double-stranded joining DNA fragment containing a sequence A and/or a sequence B is selectively joined to the ends of a target gene fragment. A mixture of a double-stranded gene fragment, the 3? end of which is protruding, and the double-stranded joining DNA fragment, which are related in a prescribed manner, undergoes at least two cycles of thermal denaturation, reassociation, and DNA synthesis, resulting in a “joined DNA fragment,” which is a double-stranded DNA fragment including at least one instance of a sequence resulting from joining sequence A, the target gene sequence, and sequence B. A “single-side joined DNA fragment” can also be obtained, by a similar method.Type: GrantFiled: September 2, 2010Date of Patent: April 7, 2015Assignee: National University Corporation University of ToyamaInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Patent number: 8993241Abstract: Provided is a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate. The projecting barrier has a cutoff portion and an inner space capable of holding a droplet and having a contact angle to pure water of from 90 to 150 degrees. A reaction method using the reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet by means of a magnet located on the opposite surface of the substrate to thereby conduct reactions and washings.Type: GrantFiled: January 16, 2009Date of Patent: March 31, 2015Assignee: National University Corporation University of ToyamaInventors: Masaharu Isobe, Nobuyuki Kurosawa
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Patent number: 8841094Abstract: The present invention provides a method which can achieve the homologous recombination of a gene of interest selectively, and a recombinant DNA molecule produced by the method. A homologous recombination method which uses a PCR product and a linearized vector is disclosed. The PCR product comprises a sequence for a target gene and amplification primer sequences P1 and P2 on both terminal ends. The vector contains homologous recombination regions VP1 and VP2 which respectively comprise nucleotide sequences homologous to P1 and P2, and at least one a homologous recombination region VT (VT1 and/or VT2), which comprises a nucleotide sequence homologous to a sequence T (T1 and/or T2), T sequences are sequence parts internal to P1 and/or P2 as well as sequence parts on the terminal side of VP1 and/or VP2 (provided that at least one T sequence has a nucleotide sequence specific to the target gene).Type: GrantFiled: March 6, 2009Date of Patent: September 23, 2014Assignee: National University Corporation University of ToyamaInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20140031528Abstract: [Problem] To provide a method that efficiently produces antigen-specific monoclonal antibodies from a wide range of animal species, and to provide a new antigen-specific monoclonal antibody using this technique. [Solution] A nonhuman animal is immunized with a target antigen, lymph fluid or the like is collected from the immunized nonhuman animal, or lymph fluid or the like is collected from a human having antibodies to the target antigen, the collected lymph fluid or the like is combined with (1) a labeled target antigen and (2) a marker that can selectively binds to plasma cells and/or plasmablasts, and cells that have bound to (1) the labeled target antigen and (2) the marker are then selected.Type: ApplicationFiled: March 28, 2012Publication date: January 30, 2014Applicant: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20130029325Abstract: A method which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker is provided. Further disclosed is a fluorescent probe wherein the staining selectivity for the endoplasmic reticulum of cells is higher than the staining selectivity for cell organelles other than the endoplasmic reticulum. Also disclosed is a method for identifying plasma cells and plasmablasts which includes staining cells derived from lymph node tissue or similar by using this probe, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells. Also disclosed is a fluorescent probe wherein the staining selectivity for cell nuclei is higher than the staining selectivity for cell organelles other than the cell nuclei.Type: ApplicationFiled: March 22, 2011Publication date: January 31, 2013Applicant: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20130023009Abstract: Provided is a method for selectively obtaining, for a given target gene, a “joined DNA fragment” wherein just a target gene fragment is joined with desired other DNA fragments, regardless of whether a DNA fragment containing a target gene sequence has been purified. In the provided method, a double-stranded joining DNA fragment containing a sequence A and/or a sequence B is selectively joined to the ends of a target gene fragment. A mixture of a double-stranded gene fragment, the 3? end of which is protruding, and the double-stranded joining DNA fragment, which are related in a prescribed manner, undergoes at least two cycles of thermal denaturation, reassociation, and DNA synthesis, resulting in a “joined DNA fragment,” which is a double-stranded DNA fragment including at least one instance of a sequence resulting from joining sequence A, the target gene sequence, and sequence B. A “single-side joined DNA fragment” can also be obtained, by a similar method.Type: ApplicationFiled: September 2, 2010Publication date: January 24, 2013Applicant: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20120161833Abstract: Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed. A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.Type: ApplicationFiled: December 10, 2011Publication date: June 28, 2012Inventors: Satoshi Yoshida, Nobuyuki Kurosawa, Kenta Sasaki
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Publication number: 20110117609Abstract: Disclosed is a method which can achieve the homologous recombination of a gene of interest selectively. Also disclosed is a recombinant DNA molecule produced by the method. Specifically disclosed is a homologous recombination method which uses a PCR product and a linearized vector. The PCR product comprises a sequence for a target gene and amplification primer sequences P1 and P2 attached to both termini of the sequence for the target gene.Type: ApplicationFiled: March 6, 2009Publication date: May 19, 2011Applicant: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMAInventors: Nobuyuki Kurosawa, Masaharu Isobe
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Publication number: 20110020879Abstract: The present invention provides a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution and the like in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate, wherein the projecting barrier has at least one cutoff portion and an inner space capable of holding a droplet, and at least the face holding the droplets of the substrate surface has a contact angle to pure water of from 90 to 150 degrees.Type: ApplicationFiled: January 16, 2009Publication date: January 27, 2011Applicant: National University Corporation University of ToyamaInventors: Masaharu Isobe, Nobuyuki Kurosawa
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Patent number: 6218161Abstract: Novel GalNAc&agr;2,6-sialyltransferases P-B1 and P-B3; GalNAc&agr;2,6-sialyltransferase genes encoding the above GalNAc&agr;2,6-sialyltransferases P-B1 and P-B3; and an extracellularly releasable protein catalyzing GalNAc&agr;2,6-sialic acid transfer which comprises a polypeptide portion as being an active domain of the GalNAc&agr;2,6-sialyltransferase P-B1 or P-B3 together with a signal peptide are provided. Also provided is a process for preparing a sialyltransferases which enables efficient recovery of a sialyltransferase expressed in a large quantity in microorganisms.Type: GrantFiled: August 28, 1998Date of Patent: April 17, 2001Assignee: The Institute of Physical and Chemical ResearchInventors: Shuichi Tsuji, Nobuyuki Kurosawa, Toshiro Hamamoto, Young-Choon Lee, Takashi Nakaoka, Naoya Kojima
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Patent number: 6017743Abstract: The present invention provides a nucleotide sequence encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase and an enzymatically active fragment thereof. The present invention also provides a nucleotide sequence encoding an extracellularly releasable protein capable of catalyzing Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransfer which comprises an enzymatically active fragment of the Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase together with a nucleotide sequence encoding at least one signal peptide. The present invention also provides recombinant vectors comprising nucleotide sequences encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase. Processes for preparing extracellularly releasable polypeptide fragments of Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase are provided as well.Type: GrantFiled: October 24, 1997Date of Patent: January 25, 2000Assignee: The Institute of Physical and Chemical ResearchInventors: Shuichi Tsuji, Yukiko Yoshida, Naoya Kojima, Nobuyuki Kurosawa, Toshiro Hamamoto