Abstract: The present disclosure relates generally to methods for the modification of plant cells, e.g., cannabis plant cells, using RNA interference (RNAi), and cells that have been modified to express mediators of RNAi, e.g., short interfering RNA (siRNA). The present disclosure further relates to plants comprising the modified cells and products derived from the plants, e.g., plant material and extracts derived therefrom and methods for selecting siRNA for altering the expression of one or more cannabinoid biosynthesis genes, such as THCAS, CBCAS or CBDAS.

Abstract: The present disclosure relates generally to targeted gene editing constructs, including methods of designing a DNA-recognition moiety for modulation of gene expression in plants, DNA-recognition moieties, gene editing constructs, methods for the modulation of gene expression in plants using gene editing constructs, and plants or regenerable plant cells produced therefrom.

Abstract: The present disclosure relates generally to methods useful to the production of cannabis plants, including methods for determining the sex of a cannabis plant, methods for determining the developmental stage of a female cannabis plant inflorescence, methods for monitoring the development of female cannabis plant inflorescence, methods for standardising the harvesting of female cannabis plants, methods for selecting a female cannabis plant for harvest and methods for selecting a hypoallergenic cannabis plant.

Abstract: The present disclosure relates to methods of determining the terpene profile of cannabis plant material, specifically through use of gas chromatography-mass spectrometry (GC-MS). Extraction methods include liquid based extraction methods, specifically hexane based liquid extraction methods, and headspace extraction methods, specifically static headspace extraction and headspace solid phase microextraction (HS-SPME). Static headspace and HS-SPME are shown to be better for monoterpene extraction, and hexane based liquid extraction for sesquiterpene extraction.

Abstract: The present invention relates generally to an artificial plant seed and to its use for plant propagation, in particular, for the propagation of cannabis plants.

Abstract: The present invention relates to determining the chemotype profile of cannabis plant material through determining cannabinoid content of the plant material using near infrared spectroscopy. The invention also involves the training of a classifier to determine the chemotype profile of a cannabis plant from the spectroscopic data.

Abstract: The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Poaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. The present invention also relates to expression of self-gamete recognition genes in plants and to related nucleic acids, constructs, molecular markers and methods.

Abstract: The present disclosure relates generally to new cannabis plants, including parts, extracts and uses thereof, comprising a cannabinoid profile enriched for total THC (i.e., ?-9-tetrahydrocannabinol (THC) and ?-9-tetrahydrocannabinolic acid (THCA), total CBG (i.e., cannabigerol (CBG) and cannabigerolic acid (CBGA)), and total THCV (i.e., tetrahydrocannabivarin (THCV) and tetrahydrocannabivarinic acid (THCVA)).

Abstract: The present disclosure relates to new Cannabis plants, including parts, extracts and uses thereof, comprising a cannabinoid profile enriched for total THC (i.e., ?-9-tetrahydrocannabinol (THC) and ?-9-tetrahydrocannabinolic acid (THCA)) and total CBG (i.e., cannabigerol (CBG) and cannabigerolic acid (CBGA)).

Abstract: The present disclosure relates generally to new Cannabis plants, including parts, extracts and uses thereof, comprising a cannabinoid profile enriched for total CBD (i.e., cannabidiol (CBD) and cannabidiolic acid (CBDA)).

Abstract: The present disclosure relates generally to new cannabis plants, including parts, extracts and uses thereof, comprising a cannabinoid profile enriched for total CBD (i.e., cannabidiol (CBD) and cannabidiolic acid (CBDA)) and for total THC (i.e., ?-9-tetrahydrocannabinol (THC) and ?-9-tetrahydrocannabinolic acid (THCA)).

Abstract: The present invention relates to medicinal cannabis plants, and cannabis plant-derived products. In particular, the present invention relates to medicinal cannabis plants having a desired cannabinoid content, methods of selecting cannabis plants having a desired cannabinoid content, chemotype and/or sex, extraction therefrom, and uses thereof. The present invention also relates to genetic markers for identifying and selecting cannabis plants having a desired chemotype and/or sex and uses thereof.

Abstract: The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organsims and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts seleced from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont.

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5? and 3? ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract: The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Poaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. The present invention also relates to expression of self-gamete recognition genes in plants and to related nucleic acids, constructs, molecular markers and methods.