Patents by Inventor Norihiko Misawa

Norihiko Misawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9963731
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: May 8, 2018
    Assignee: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Publication number: 20170191103
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Application
    Filed: December 20, 2016
    Publication date: July 6, 2017
    Applicant: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Patent number: 9562220
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Grant
    Filed: June 8, 2012
    Date of Patent: February 7, 2017
    Assignee: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Publication number: 20140170700
    Abstract: The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
    Type: Application
    Filed: June 8, 2012
    Publication date: June 19, 2014
    Applicant: National University Corporation Chiba University
    Inventors: Daisuke Umeno, Maiko Furubayashi, Norihiko Misawa, Shinichi Takaichi
  • Patent number: 7999151
    Abstract: To provide a microorganism or a plant transformed with a ?-ionone ring-4-ketolase gene and/or ?-ionone ring-3-hydroxylase gene derived from Brevundimonas sp. strain SD-212. The ?-ionone ring-4-ketolase gene and ?-ionone ring-3-hydroxylase gene produced by Brevundimonas sp. strain SD-212 each have a high activity compared with those of known enzymes, and therefore microorganisms transformed with the genes encoding these enzymes can efficiently produce astaxanthin.
    Type: Grant
    Filed: May 26, 2005
    Date of Patent: August 16, 2011
    Assignee: Kirin Holdings Kabushiki Kaisha
    Inventors: Seon-Kang Choi, Norihiko Misawa
  • Patent number: 7695931
    Abstract: The present invention provides a Brevundimonas sp. strain SD212-derived peptide having ?-ionone ring-2-hydroxylase activity and a gene encoding the same, to thereby make it possible to produce rare carotenoids in which a hydroxyl group is introduced at the position 2(2?) carbon in their ?-ionone ring in large quantities. The present invention also provides a novel gene encoding an enzyme which introduces a hydroxyl group at the position 3(3?) carbon in the ?-ionone ring of carotenoids, and a novel gene encoding a geranylgeranyl pyrophosphate synthase.
    Type: Grant
    Filed: November 4, 2004
    Date of Patent: April 13, 2010
    Assignee: Kirin Holdings Kabushiki Kaisha
    Inventors: Yasuhiro Nishida, Kyoko Adachi, Hiroaki Kasai, Yoshikazu Shizuri, Sadao Komemushi, Norihiko Misawa
  • Publication number: 20090298146
    Abstract: To provide a microorganism or a plant transformed with a ?-ionone ring-4-ketolase gene and/or ?-ionone ring-3-hydroxylase gene derived from Brevundimonas sp. strain SD-212. The ?-ionone ring-4-ketolase gene and ?-ionone ring-3-hydroxylase gene produced by Brevundimonas sp. strain SD-212 each have a high activity compared with those of known enzymes, and therefore microorganisms transformed with the genes encoding these enzymes can efficiently produce astaxanthin.
    Type: Application
    Filed: May 26, 2005
    Publication date: December 3, 2009
    Applicant: MARINE BIOTECHNOLOGY INSTITUTE CO., LTD.
    Inventors: Seon-Kang Choi, Norihiko Misawa
  • Publication number: 20080254519
    Abstract: The present invention relates to a method for producing a picolinic acid compound. Specifically, the present invention relates to a method for producing a picolinic acid compound, which comprises reacting an aromatic compound that contains a phenyl group represented by the following formula (I), (II), or (III) with aromatic ring dioxygenase, aromatic ring dihydrodiol dehydrogenase, and aromatic ring diol dioxygenase, and obtaining a picolinic acid compound (I?), (II?), or (III?). wherein, H1 is an optionally substituted heterocyclic group, A1 is a single bond or an optionally substituted C1-4 alkylene group or alkenylene group, P2 is an optionally substituted phenyl group, and C1 is an optionally substituted cyclic hydrocarbon group (excluding a phenyl group), and where formula II does not represent diphenylacetylene.
    Type: Application
    Filed: February 22, 2005
    Publication date: October 16, 2008
    Inventors: Kazutoshi Shindo, Osamu Kagami, Norihiko Misawa, Kensuke Furukawa
  • Publication number: 20080220419
    Abstract: The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources. The present invention further provides a fused cytochrome P450 monooxygenase containing a peptide which is linked to the C-terminus of a P450 protein with a linker portion disposed therebetween and which has the same function as that of a reductase domain contained in a cytochrome P450 monooxygenase originating from Rhodococcus sp. strain NCIMB 9784.
    Type: Application
    Filed: November 2, 2005
    Publication date: September 11, 2008
    Applicant: Marine Biotechnology Institute Co., Ltd.
    Inventors: Mitsutoshi Kubota, Miho Nodate, Kenichi Nodate, Taku Uchiyama, Norihiko Misawa
  • Publication number: 20070148727
    Abstract: The present invention provides a Brevundimonas sp. strain SD212-derived peptide having ?-ionone ring-2-hydroxylase activity and a gene encoding the same, to thereby make it possible to produce rare carotenoids in which a hydroxyl group is introduced at the position 2 (2?) carbon in their ?-ionone ring in large quantities. The present invention also provides a novel gene encoding an enzyme which introduces a hydroxyl group at the position 3 (3?) carbon in the ?-ionone ring of carotenoids, and a novel gene encoding a geranylgeranyl pyrophosphate synthase.
    Type: Application
    Filed: November 4, 2004
    Publication date: June 28, 2007
    Applicant: Marine Biotechnology Institute Co.,Ltd
    Inventors: Yasuhiro Nishida, Kyoko Adachi, Yoshikazu Shizuri, Hiroaki Kasai, Sadao Komemushi, Norihiko Misawa
  • Patent number: 6821749
    Abstract: The production of carotenoid is accomplished using a DNA molecule that encodes a polypeptide as obtained from Haematococcus pluvialis, Phaffia rhodozyma, or Saccharomyces cerevisiae, having isopentonyl pyrophosphate (IPP) isomerase activity, or DNA molecule having a nucleotide sequence that hybridizes thereto. In particular, one can introduce such a DNA molecule into a carotenoid-producing microorganism, culture the microorganism thus transformed, and then obtain carotenoids in the culture broth and cells.
    Type: Grant
    Filed: November 12, 1996
    Date of Patent: November 23, 2004
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Susumu Kajiwara, Norihiko Misawa, Keiji Kondo
  • Publication number: 20040086983
    Abstract: An objective of the present invention is to provide a method of producing hydroxylated heterocyclic compounds and hydroxylated aromatic carboxylic acids by bioengineering technique, and modified enzymes which can be used for this method. A method of producing hydroxylated heterocyclic compounds or hydroxylated aromatic carboxylic acids comprises reacting an aromatic ring dioxygenase with heterocyclic compounds or aromatic carboxylic acids to hydroxylate these compounds. An enzyme according to the present invention is an aromatic ring dioxygenase comprising an &agr;-subunit consisting of the amino acid sequence of SEQ ID NO: 2, which is modified according to the &agr;-subunit of the biphenyl dioxygenase derived from the strain Burkholderia cepacia LB400, a &bgr;-subunit consisting of the amino acid sequence of SEQ ID NO: 4, and a ferredoxin consisting of the amino acid sequence of SEQ ID NO: 6, and a ferredoxin reductase consisting of the amino acid sequence of SEQ ID NO: 8.
    Type: Application
    Filed: September 13, 2001
    Publication date: May 6, 2004
    Inventors: Norihiko Misawa, Kazutoshi Shindo, Hiroshi Okazaki, Kensuke Furukawa, Sueharu Horinouchi
  • Patent number: 6150130
    Abstract: Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
    Type: Grant
    Filed: June 18, 1999
    Date of Patent: November 21, 2000
    Assignees: Kirin Beer Kabushiki Kaisha, Marine Biotechnology Institute Co., Ltd.
    Inventors: Norihiko Misawa, Keiji Kondo, Susumu Kajiwara, Akihiro Yokoyama
  • Patent number: 5972690
    Abstract: Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptides having and enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
    Type: Grant
    Filed: January 13, 1998
    Date of Patent: October 26, 1999
    Assignees: Kirin Beer Kabushiki Kaisha, Marine Biotechnology Institute Co., Ltd
    Inventors: Norihiko Misawa, Keiji Kondo, Susumu Kajiwara, Akihiro Yokoyama
  • Patent number: 5910433
    Abstract: DNA sequences are described that encode genes for synthesizing ketocarotenoids such as astaxanthin. The DNA sequences, microorganisms containing them and encoded polypeptides are described. Also described are methods to obtain related sequences and to make host cells that contain such sequences. These genes and methods are useful to impart red coloration during culture of fish and crustaceans, as coloring in food, and as antioxidants.
    Type: Grant
    Filed: April 23, 1996
    Date of Patent: June 8, 1999
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Susumu Kajiwara, Norihiko Misawa, Keiji Kondo
  • Patent number: 5849524
    Abstract: An reproducible transformation system of a yeast of Candida utilis, a process for expressing a heterologous gene in the transformation system, a vector which can be used in the transformation system and the expression method, and a novel DNA group are disclosed. In particular, the process for expressing a heterologous gene in Candida utilis comprises transforming Candida utilis with a vector comprising a drug-resistance marker, a sequence homologous to the chromosomal DNA of the Candida utilis yeast, and the heterologous gene, culturing the transformant, and isolating the expression product of the heterologous gene.
    Type: Grant
    Filed: May 24, 1996
    Date of Patent: December 15, 1998
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Keiji Kondo, Susumu Kajiwara, Norihiko Misawa
  • Patent number: 5811273
    Abstract: Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
    Type: Grant
    Filed: September 23, 1996
    Date of Patent: September 22, 1998
    Assignees: Kirin Beer Kabushiki Kaisha, Marine Biotechnology Institute Co., Ltd,
    Inventors: Norihiko Misawa, Keiji Kondo, Susumu Kajiwara, Akihiro Yokoyama
  • Patent number: 5589581
    Abstract: Disclosed are DNA sequences which are useful for the synthesis of carotenoids such as lycopene, .beta.-carotene, zeaxanthin or zeaxanthin-diglucoside, that is, DNA sequences encoding carotenoid biosynthesis enzymes. These DNA sequences are the sequences 1- 6, respectively, shown in the specification.Also disclosed is a process for producing a carotenoid compound which is selected from the group consisting of prephytoene pyrophosphate, phytoene, lycopene, .beta.-carotene, zeaxanthin and zeaxanthin-diglucoside, which comprises transforming a host with at least one of the DNA sequences 1- 6 described above and culturing the transformant.
    Type: Grant
    Filed: March 10, 1994
    Date of Patent: December 31, 1996
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Norihiko Misawa, Kazuo Kobayashi, Katsumi Nakamura
  • Patent number: 5429939
    Abstract: Disclosed are DNA sequences which are useful for the synthesis of carotenoids such as lycopene, .beta.-carotene, zeaxanthin or zeaxanthin-diglucoside, that is, DNA sequences encoding carotenoid biosynthesis enzymes. These DNA sequences are the sequences 1-6 shown in the specification.Also disclosed is a process for producing a carotenoid or a carotenoid related compound which is selected from the group consisting of geranylgeranyl pyrophosphate, phytoene, lycopene, .beta.-carotene, zeaxanthin and zeaxanthin-diglucoside, which comprises transforming a host with at least one of the DNA sequences 1-6 described above and culturing the transformant.
    Type: Grant
    Filed: October 23, 1991
    Date of Patent: July 4, 1995
    Assignee: Kirin Beer Kabushiki Kaisha
    Inventors: Norihiko Misawa, Kazuo Kobayashi, Katsumi Nakamura, Shigeyuki Yamano