Abstract: A pharmaceutical includes helper T cells induced from pluripotent stem cells. The helper T cells include CD4-positive CD40L-highly expressing T cells, dendritic cells, antigen, and cytotoxic T cells CD8-positive T cells. The pharmaceutical can be administered in a method for treating cancer to a patient having cancer cells expressing an antigen specifically recognized by CD4-positive T cells.

Abstract: A method of producing helper T cells, comprising: (i) culturing T cells, which have been induced from pluripotent stem cells and into which a CD4 gene or a gene product thereof has been introduced, in a medium containing IL-2 and IL-15; and (ii) isolating CD40L-highly expressing T cells from cells obtained in step (i).

Abstract: A voltage follower circuit according to an embodiment includes first and second paths, the first path includes a first nMOS transistor and a first pMOS transistor, the second path includes a second nMOS transistor and a second pMOS transistor, an input voltage is supplied to the gate of the first nMOS transistor, an output voltage is supplied to the gate of the second nMOS transistor, a voltage lower than the output voltage is supplied to the gate of the first pMOS transistor, and a voltage lower than the input voltage is supplied to the gate of the second pMOS transistor.

Abstract: A method of producing helper T cells, comprising: (i) culturing T cells, which have been induced from pluripotent stem cells and into which a CD4 gene or a gene product thereof has been introduced, in a medium containing IL-2 and IL-15; and (ii) isolating CD40L-highly expressing T cells from cells obtained in step (i).

Abstract: A comparator includes a differential pair circuit comprising NMOS transistors, the differential pair circuit configured to output a signal corresponding to a difference between first and second input signals supplied thereto, and an input circuit configured to raise a voltage level of the first input signal supplied to the differential pair circuit when the voltage of the first input signal is less than a predetermined threshold value.

Abstract: A voltage follower circuit according to an embodiment includes first and second paths, the first path includes a first nMOS transistor and a first pMOS transistor, the second path includes a second nMOS transistor and a second pMOS transistor, an input voltage is supplied to the gate of the first nMOS transistor, an output voltage is supplied to the gate of the second nMOS transistor, a voltage lower than the output voltage is supplied to the gate of the first pMOS transistor, and a voltage lower than the input voltage is supplied to the gate of the second pMOS transistor.

Abstract: A technique for accurately detecting a temperature of a sealing surface which is not affected by sealing conditions or the like is provided. A seal bar (1) is a rod-shaped seal bar having a sealing surface (11a) and includes a heater (13) and a temperature sensor (14). The heater (13) is provided in a main body of the seal bar (1) and extends in an extending direction (D1) of the main body (11). The temperature sensor (14) is provided at a position between the sealing surface (11a) and the heater (13) in the main body (11) of the seal bar (1) and is inserted into the main body (11) of the seal bar (1) from an end surface (upper end surface (112) or the like) which intersects a long side of the sealing surface (11a).

Abstract: A regulator amplifier circuit of an embodiment includes a differential amplifier circuit, an nMOS transistor, and a pMOS transistor. The differential amplifier circuit includes a differential circuit and a transistor. The differential circuit includes a differential MOS transistor circuit, and the transistor includes a gate voltage controlled by the differential circuit. The nMOS transistor includes a drain connected to a drain on minus side of the differential MOS transistor, and a gate connected to a source of the transistor. The nMOS transistor operates in a weak inversion region. The pMOS transistor includes a source connected to a source of the nMOS transistor, and a drain connected to a voltage lower than a source voltage of the nMOS transistor. The pMOS transistor operates in the weak inversion region.

Abstract: A regulator amplifier circuit of an embodiment includes a differential amplifier circuit, an nMOS transistor, and a pMOS transistor. The differential amplifier circuit includes a differential circuit and a transistor. The differential circuit includes a differential MOS transistor circuit, and the transistor includes a gate voltage controlled by the differential circuit. The nMOS transistor includes a drain connected to a drain on minus side of the differential MOS transistor, and a gate connected to a source of the transistor. The nMOS transistor operates in a weak inversion region. The pMOS transistor includes a source connected to a source of the AMOS transistor, and a drain connected to a voltage lower than a source voltage of the nMOS transistor. The pMOS transistor operates in the weak inversion region.

Abstract: An object of the present invention is induction of CD4-positive T cells from pluripotent stem cells. This object is achieved by production of CD4-positive T cells by introducing a CD4 gene or a gene product thereof into T cells induced from pluripotent stem cells.

Abstract: A comparator includes a differential pair circuit comprising NMOS transistors, the differential pair circuit configured to output a signal corresponding to a difference between first and second input signals supplied thereto, and an input circuit configured to raise a voltage level of the first input signal supplied to the differential pair circuit when the voltage of the first input signal is less than a predetermined threshold value.

Abstract: A technique for accurately detecting a temperature of a sealing surface which is not affected by sealing conditions or the like is provided. A seal bar (1) is a rod-shaped seal bar having a sealing surface (11a) and includes a heater (13) and a temperature sensor (14). The heater (13) is provided in a main body of the seal bar (1) and extends in an extending direction (D1) of the main body (11). The temperature sensor (14) is provided at a position between the sealing surface (11a) and the heater (13) in the main body (11) of the seal bar (1) and is inserted into the main body (11) of the seal bar (1) from an end surface (upper end surface (112) or the like) which intersects a long side of the sealing surface (11a).

Abstract: The reference circuit includes a first switch element that is connected to the first node at a first end of a current path thereof. The reference circuit includes a first capacitive element that is connected to a second end of the current path of the first switch element at a first end thereof and to a fixed potential at a second end thereof. The reference circuit includes an output circuit that outputs a third voltage that is based on a second voltage at the first end of the first capacitive element. The reference circuit includes a comparator that compares a fourth voltage correlated with the second voltage and a fifth voltage and outputs a comparison result signal in accordance with a result of the comparison. The reference circuit includes a controlling circuit that controls operation of the voltage generating circuit and the first switch element.

Abstract: The step-down regulator includes a first error amplifying circuit that receives a first reference voltage and the first voltage and supplies a first control signal to a control terminal of the first transistor so that the first reference voltage and the first voltage are equal to each other. The step-down regulator includes a second error amplifying circuit that receives a voltage at the second end of the current controlling circuit and a second reference voltage and supplies a second control signal to the current controlling circuit so that the voltage at the second end of the current controlling circuit and the second reference voltage are equal to each other. The step-down regulator includes a diode that is connected to the second end of the current controlling circuit at an anode thereof and to the second potential at a cathode thereof.

Abstract: The step-down regulator includes a first error amplifying circuit that receives a first reference voltage and the first voltage and supplies a first control signal to a control terminal of the first transistor so that the first reference voltage and the first voltage are equal to each other. The step-down regulator includes a second error amplifying circuit that receives a voltage at the second end of the current controlling circuit and a second reference voltage and supplies a second control signal to the current controlling circuit so that the voltage at the second end of the current controlling circuit and the second reference voltage are equal to each other. The step-down regulator includes a diode that is connected to the second end of the current controlling circuit at an anode thereof and to the second potential at a cathode thereof.

Abstract: Provided are crystals of compound A which have properties suitable for industrial production. MEANS FOR SOLVING As the results of intensive studies to provide crystals of compound A having an inhibitory activity on the kinase activity of an EML4-ALK fusion protein and a mutant EGFR protein, crystals of compound A were found. Moreover, it was found that A04-type crystal of compound A, from among the aforesaid crystals of compound A, unexpectedly have preferred properties as a drug substance.

Abstract: The present invention provides isolated DNA encoding a GWT1 protein having activity to confer resistance of a fungus against a compound of formula Ia, and wherein a defect of a function of the GWT1 protein leads to a decrease in the amount of a glycosylphosphatidylinositol (GPI)-anchored protein in the cell wall of a fungus.

Abstract: The present invention provides an antifungal agent represented by the formula: wherein A1 represents a 3-pyridyl group which may have a substituent, a quinolyl group which may have a substituent, or the like; X1 represents a group represented by the formula —NH—C(?O)—, a group represented by the formula —C(?O)—NH—, or the like; E represents a furyl group, a thienyl group, a pyrrolyl group, a phenyl group, a pyridyl group, a tetrazolyl group, a thiazolyl group or a pyrazolyl group; with the proviso that A1 may have 1 to 3 substituents, and E has one or two substituents.

Abstract: The present invention provides an antifungal agent represented by the formula: [wherein A1 represents a 3-pyridyl group which may have a substituent, a quinolyl group which may have a substituent, or the like; X1 represents a group represented by the formula —NH—C(?O)—, a group represented by the formula —C(?O)—NH—, or the like; E represents a furyl group, a thienyl group, a pyrrolyl group, a phenyl group, a pyridyl group, a tetrazolyl group, a thiazolyl group or a pyrazolyl group; with the proviso that A1 may have 1 to 3 substituents, and E has one or two substituents].

Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.