Abstract: A method is provided for expressing a foreign gene, comprising the steps of: (a) producing a plant which functionally expresses an RNA1 genome and/or an RNA2 genome of Cucumber Mosaic Virus (CMV); (b) introducing a nucleic acid vector into the plant, said nucleic acid vector comprising, arranged in this order: (i) a right border sequence (RB) derived from the Agrobacterium T-DNA sequence; (ii) an expression promoter sequence workable in the plant; (iii) a sequence corresponding to an RNA3 genome of CMV in which a 3b gene, which codes for a 3b protein (coat protein), of CMV has been prevented from being expressed and the foreign gene has been inserted downstream of, and operably linked with, a subgenomic promoter which otherwise promotes the expression of the 3b gene; and (iv) a left border sequence (LB) derived from the Agrobacterium T-DNA sequence; and (c) culturing the plant containing the nucleic acid vector such that the RNA1 and/or RNA2 genome(s) of CMV is functionally expressed by the plant while th

Abstract: A nucleic acid molecule for expressing a foreign gene in a plant, said nucleic acid molecule carrying, in this order, a right border (RB) sequence derived from the T-DNA sequence of an agrobacterium, an expression promoter sequence capable of functioning in the aforesaid plant, a sequence corresponding to the RNA2 genome of cucumber mosaic virus wherein a 2b protein-encoding gene has been partly or entirely substituted by the aforesaid foreign gene, and a left border (LB) sequence derived from the T-DNA sequence of an agrobacterium.

Abstract: The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transformed with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transformed with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.

Abstract: The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transfected with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transfected with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.