Abstract: It is an objective of the present invention to develop a Grifola-derived substance having advanced immunopotentiating activity and antitumor activity, which has a molecular weight lower than that of a conventionally known glucan-protein complex and is structurally different from such complex. A Grifola-derived low-molecular-weight substance is produced by the following steps 1) to 4): 1) a step of thermally extracting mycelia or fruit bodies of Grifola with water; 2) a step of adding alcohol to the obtained water-soluble extract fraction to a final concentration between 10% and 80% by volume, allowing the resulting solution to stand at a temperature between 1° C. and 40° C.

Abstract: It is an objective of the present invention to develop a Grifola-derived glucan-protein complex that has a molecular weight lower than that of a conventionally known high-molecular-weight glucan-protein complex and has advanced immunopotentiating activity and antitumor activity. The glucan-protein complex of interest was obtained through the following 1) to 5): 1) a step of thermally extracting mycelia or fruit bodies of Grifola with water; 2) a step of adding alcohol to the obtained water-soluble extract fraction to a final concentration between 20% and 70% by volume, allowing the resulting solution to stand at a temperature between 1° C. and 25° C.