Abstract: The invention provides a rose characterized by comprising a flavone and delphinidin added by a genetic modification method. The flavone and delphinidin are typically produced by expression of a transferred flavone synthase gene and flavonoid 3?,5?-hydroxylase gene, respectively. The flavone synthase gene is, for example, a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3?,5?-hydroxylase gene is, for example, the pansy (Viola×wittrockiana) flavonoid 3?,5?-hydroxylase gene.

Abstract: The invention provides a rose characterized by comprising a flavone and malvidin added by a genetic modification method. The flavone and malvidin are typically produced by expression of a transferred flavone synthase gene, pansy flavonoid 3?,5?-hydroxylase gene and anthocyanin methyltransferase gene. The flavone synthase gene is, for example a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3?,5?-hydroxylase gene is, for example, the pansy flavonoid 3?,5?-hydroxylase gene. The anthocyanin methyltransferase gene is, for example, the methyltransferase gene of torenia of the family Scrophulariaceae.

Abstract: There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4?CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

Abstract: There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4?CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

Abstract: Disclosed is a method for determining whether or not a wild rose of interest is crossed with a cultivated rose. The method comprises the steps of: examining whether or not a KSN gene containing a transposon (an indicator) is contained in the rose of interest; and determining that the rose of interest is crossed with a cultivated rose when the individual has the transposon-containing KSN gene.

Abstract: The present invention provides SELF protein having controlling effects on growth and differentiation of undifferentiated cells, wherein the protein contains a novel EGF-like repeat sequence, SELF gene encoding the same, a recombinant vector and a transformed cell containing the SELF gene, a method for treatment or prophylaxis with SELF protein or a recombinant expression vector containing SELF gene, SELF promoter, a recombinant vector and a transformed cell containing SELF promoter, and a screening method using the transformed cell containing SELF promoter.

Abstract: The present invention provides SELF protein having controlling effects on growth and differentiation of undifferentiated cells, wherein the protein contains a novel EGF-like repeat sequence, SELF gene encoding the same, a recombinant vector and a transformed cell containing the SELF gene, a method for treatment or prophylaxis with SELF protein or a recombinant expression vector containing SELF gene, SELF promoter, a recombinant vector and a transformed cell containing SELF promoter, and a screening method using the transformed cell containing SELF promoter.

Abstract: The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2? of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2? of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2? of the chalcone, i.e., the glycosyltransferase activity at position 2? of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2?.

Abstract: There is provided a gene coding for the amino acid sequence listed as SEQ ID NO: 2 or SEQ ID NO: 70, for example. Co-expression of the 4?CGT gene and AS gene in a plant lacking natural aurone synthesis ability is carried out to successfully accumulate aurones and alter the flower color to have a yellow tint. In addition to the expression of both genes, the flavonoid pigment synthesis pathway of the host plant itself is inhibited to obtain flowers with a more defined yellow color.

Abstract: The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2? of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2? of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2? of the chalcone, i.e., the glycosyltransferase activity at position 2? of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2?.

Abstract: Disclosed are a equine interleukin-1 peptide which comprises a sequence comprising at least 5 continuous amino acids in a peptide represented by SEQ ID NO: 1 or NO: 2 in the sequence listing, DNA coding the equine interleukin-1 peptide, a recombinant vector containing the DNA, a transformant containing the recombinant vector, and a process for preparing an anti-equine interleukin-1 antibody, which comprises using the equine interleukin-1 peptide as an antigen.

Abstract: An object of the present invention is to provide a method for manufacturing a magnetic hard disk wherein a magnetic material layer adhered on a surface of a substrate of the magnetic hard disk has a flat surface.A method for manufacturing a magnetic hard disk comprising steps of: structurally transforming a surface of an amorphous Ni--P alloy film layer formed a substrate (10) of the magnetic hard disk; and adhering a magnetic material layer on the surface of a substrate (10), wherein the step of structurally transforming the a-Ni--P alloy film layer is a step of heating only a desired portion of the surface of the substrate (10) by an electric discharge to generate Ni and crystallized compounds of Ni and P only at the desired portion (T) of the surface thereof.