Abstract: The present invention provides a hybridization detection method capable of quantitatively determining the degree of the hybridization between a sample biopolymer and a probe biopolymer. In the method, the amount of a fluorescently labeled probe immobilized on a substrate as a spot is quantitatively determined by determining the fluorescence emitted from a fluorescent material labeling the probe, and the amount of a fluorescently labeled sample hybridized to the probe is quantitatively determined by determining the fluorescence emitted from the fluorescent material labeling the sample. The difference between the amount of the probe and the amount of the sample is normalized with the amount of the probe. Based on the normalized value, the amount of the sample hybridized to the probe can be determined relative to the amount of the probe spotted on the substrate.

Abstract: The present invention allows detection in short time while maintaining the level of the resolving power as high as when a capillary is utilized. The entire surface of an electrophoresis board 10, which is provided with a two-dimensional tubular electrophoresis pathway, is irradiated with excitation light so as to pick up fluorescence or luminescence from a sample with a two-dimensional sensor. By reading the entire electrophoresis pathway, the separated state of the sample may be confirmed during the electrophoresis. As a result, the electrophoresis may be ended when the sample is sufficiently separated and a molecular weight thereof may be determined based on the migration distance.

Abstract: A coated substrate for immobilizing a biological substance etc. at high density with low background value. The substrate has a cross-linking reagent represented by general formula A—L—B (wherein, A and B represent an identical or different group which reacts with an active group of the coupling agent comprising an active group, selected from an active ester group, isothiocyanate group, isocyanate group, imidazole group, carbodiimide group or aldehyde group, and wherein L is a linking group linking A and B, selected from a straight chain alkyl group, aryl group, allyl group or alkyl group having an amide group) bound thereonto by means of a coupling agent comprising an active group. An biological substance or the like is immobilized on the substrate.

Abstract: A solution for substrate spotting and spotting method for immobilizing a biological substance or various chemical substances on a substrate at high density. The solution for substrate spotting and the spotting method involve mixing a solution comprising a biological substance or chemical substance to be spotted with a solution to which an inorganic fine powder has been added as a surface tension regulating agent.

Abstract: A device for making a biochip comprising probes spotted on a plate at a plurality of positions by using a binding agent for binding the probes to the plate, wherein the binding agent is locally spotted at positions where the probes are spotted.

Abstract: The invention uses a continuous-wave laser as an excitation light source to achieve highly-accurate detection with a simple structure. A sample 11a is irradiated with excitation light from an excitation light source 30, and then transported to an optical axis of a fluorescence detector 50, thereby being detected of its fluorescence intensity.

Abstract: A sample application method including the steps of: rotating a biochip including a plurality of regions bound with various probes; and dropping a sample onto generally a center of rotation of the rotating biochip so as to apply the sample to the plurality of regions.

Abstract: A method is disclosed which is capable of increasing a S/N ratio without subjecting a sample to any treatment to thereby detect hybridization with high sensitivity. A fragmentarily divided probe is used which is prepared by dividing a single probe 12 into a plurality of fragmentary probes 12a, 12b, and labeling the fragmentary probes with different fluorescent materials 14a, 14b, respectively. When the probe is hybridized with a sample 11, the fragmentary probes 12a,12b reconstitute the single probe. An intermittent light beam is used as excitation light 19. The fluorescent material 14a is excited therewith, and fluorescence 18 emitted from the fluorescent material 14b through multistep excitation is detected while irradiation with the excitation light is intermitted.

Abstract: A probe-bearing element according to the present invention includes a sheet-like body having a plurality of through holes which each bears a biopolymer probe. The sheet-like body may have a thickness of 100 .mu.m to 2 mm, and may be made of glass, acrylic resin, metal or plastic. Preferably, the size of the through hole is about 10-100 .mu.m in diameter considering the number of samples relative to the size of the element, amounts of probes required for hybridization and the detection sensitivity. The plurality of through holes are preferably arranged in concentric circles or in a spiral.

Abstract: The apparatus for reading a luminescence pattern is equipped with a stage on which the sample to be read is mounted. A photoreceptor disk is installed in the lower part of the stage and scans and condenses light from the luminescent pattern of the sample at positions determined by the rotation of a rotary plate. A transport mechanism causes the stage and the photoreceptor disk to undergo relative motion. An optical guide part guides the light from the luminescent pattern to a photoelectric converter. The optical guide part may include mirrors or one or more optical fibers. The photoelectric converter converts the light into an electrical signal. A controller controls how scanning is performed by the photoreceptor disk and transport mechanism and generates a read scanning position signal. A data processor performs data processing by converting the electrical signal into a digital signal and obtains a scanning position signal from the controller.