Abstract: Disclosed herein are an amino acid analysis method and a liquid chromatographic apparatus for improving separation performance of threonine, serine, glycine, and alanine. The method of analyzing amino acids using the liquid chromatographic apparatus equipped with a cation exchange column includes a process for distributing a sample containing threonine, serine, glycine, and alanine as the amino acids, together with an eluent, to the cation exchange column to separate threonine, serine, glycine, and alanine, wherein a column temperature when separating threonine and serine is higher than a column temperature when separating glycine and alanine.

Abstract: A liquid chromatograph includes a liquid feeding portion, a sample injection portion, a separation column, a first temperature adjustment device which raises temperature of the separation column to a first temperature, a detector, a second temperature adjustment device which is provided upstream of the separation column and adjusts temperature of a mobile phase to a second temperature lower than the first temperature, and a control device which controls at least one of the liquid feeding portion and the second temperature adjustment device. The control device controls at least one of the liquid feeding portion and the second temperature adjustment device such that the mobile phase adjusted to the second temperature is fed to the separation column before a next sample is injected after separation of sample components by the separation column ends.

Abstract: In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.

Abstract: To provide a column packing, a separation column, an analysis device, and an analysis method to reduce the analysis time without deteriorating the separation of multi-component amino acids. Disclosed are a column packing comprising a core portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 50% or more and a shell portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 20% or less; a separation column using the column packing; and a high-speed liquid chromatography analysis device and a analysis method using the separation column.

Abstract: In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.