Abstract: In a nanochannel thin film in which oxide layers have surfactant micelles therein, the presence of a target substance in a sample solution is detected with a luminescence intensity of a thin film provided by recognition of the target substance with a luminescent recognition reagent in the nanochannels. Upon focusing on a hydrophobic field provided by the presence of the surfactant in pores of a nanometer size, the novel development of a sensor function is enabled.

Abstract: A solution including a single-stranded nucleic acid (10) having a target base (11) related to SNP or the like is mixed with a solution including two kinds of single-stranded detecting nucleic acids (20a) and (20b) complementary to partial sequences that sandwich the target base (11) between them to hybridize the target nucleic acid (10) and the detecting nucleic acids (20a) and (20b). Thus, a gap part (21) is intentionally formed at a position opposed to the target base (11). Then, a receptor molecule (30) having hydrogen bonding characteristics and fluorescence emitting characteristics is inserted to the gap part (21) as a hydrophobic space. Then, the change of fluorescent strength of the receptor molecule (30) depending on the difference of the target base (11) is detected to detect a single nucleotide substitution.

Abstract: In a nanochannel thin film in which oxide layers have surfactant micelles therein, the presence of a target substance in a sample solution is detected with a luminescence intensity of a thin film provided by recognition of the target substance with a luminescent recognition reagent in the nanochannels. Upon focusing on a hydrophobic field provided by the presence of the surfactant in pores of a nanometer size, the novel development of a sensor function is enabled.