Abstract: The present invention provides a baglike container for centrifugation that is mounted in a centrifuge to thereby allow centrifugation of a dispersion liquid accommodated therein. The baglike container for centrifugation is less likely to tear or break as a result of centrifugation by disposing a container wall surface of the baglike container so as to apply centrifugal force perpendicular to the container wall surface.

Abstract: Baglike container (1) that is fitted in a centrifuge to thereby allow centrifugation of any dispersion liquid placed therein. The baglike container for centrifugation is one comprising a main frame constituted of first container wall (3) with recess portion (6) formed so as to allow fitting in a centrifuge and with flange portion (7) provided on an open face border of the recess portion (6) and constituted of flexible second container wall (4) having its peripheral portion sealed mutually with the flange portion (7) of the first container wall (3) so as to define storing space (6?) for dispersion liquid in cooperation with the recess portion (6) and further comprising port (5) fitted to the main frame in a fashion allowing communication with the storing space (6?), wherein the recess portion (6) defining the storing space (6?) can be set in the centrifuge so that centrifugal force perpendicular to the bottom plane of the recess portion (6) applies.

Abstract: A cell-preservation liquid containing at least a saccharide, sodium ion, and potassium ion, which is characterized in that the molar concentration of the contained sodium ion is equal to or more than that of the potassium ion is provided. The preservation liquid enables not only cryopreservation but also cold preservation, and an easy cell-preservation method may be provided. Moreover, when the cell-preservation liquid of the present invention is used, cell culture may be performed by inoculating cells in a medium with the cells suspended in the preservation liquid without a washing step or the like, so that the cells may be easily cultured.

Abstract: The invention relates to an agent for promoting growth in animal cells. The agent contains a serum-free medium and lysozyme. The agent can optionally contain cytokines. The invention further relates to an in vitro method of stimulating the growth of T lymphoblastoid cells and peripheral blood lymphocytes, which comprises the step of culturing the T lymphoblastoid cells or peripheral blood lymphocytes in a serum-free culture medium comprising human lysozyme, as a growth stimulating agent, at a concentration of about 4-100 mg/liter.

Abstract: An agent for stimulating growth of animal cells in vitro comprises ginseng or an active ingredient extracted from ginseng Serum-free culture media containing the agent are disclosed. The present serum-free media contain less impure protein than serum-containing media, so that isolation and purification of substances produced by animal cells cultured therein is facilitated. The present serum-free media enhance the growth of lymphocytes in vitro, and can be used for vital cell transplant therapy, particularly for adoptive immunotherapy.