Abstract: The present invention provides a means for accurately discriminating whether an onion is an onion with no pungent taste and/or tear-inducing property. The present invention relates to a method of discriminating traits of an onion, comprising a first determination step of determining presence of the nucleotide sequence of SEQ ID NO:1 corresponding to alliinase gene 1 in a nucleic acid derived from the onion, and a second determination step of determining presence of the nucleotide sequence of SEQ ID NO:2 corresponding to alliinase gene 2, wherein the onion is discriminated to be an onion with no pungent taste and/or tear-inducing property if the presence of the nucleotide sequence of SEQ ID NO:1 is not determined in the first determination step and the presence of the nucleotide sequence of SEQ ID NO:2 is determined in the second determination step.

Abstract: The present invention provides a means for accurately discriminating whether an onion is an onion with no pungent taste and/or tear-inducing property. The present invention relates to a method of discriminating traits of an onion, comprising a first determination step of determining presence of the nucleotide sequence of SEQ ID NO:1 corresponding to alliinase gene 1 in a nucleic acid derived from the onion, and a second determination step of determining presence of the nucleotide sequence of SEQ ID NO:2 corresponding to alliinase gene 2, wherein the onion is discriminated to be an onion with no pungent taste and/or tear-inducing property if the presence of the nucleotide sequence of SEQ ID NO:1 is not determined in the first determination step and the presence of the nucleotide sequence of SEQ ID NO:2 is determined in the second determination step.

Abstract: This invention provides an onion exhibiting very weak or no pungency and a method for producing such onion. In such onion, alliinase gene expression is reduced.

Abstract: This invention provides an onion exhibiting very weak or no punuency and a method for producing such onion. in such onion, alliinase gene expression is reduced.

Abstract: The present invention provides DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable.

Abstract: The present invention relates to protein or polypeptide capable of converting 1-propenylsulfenic acid concerning the formation of lachrymatory factors generated when plants such as onions are broken into pieces or cut into lachrymatory factors, and also to DNA (lachrymatory factor-producing enzyme genes) encoding the protein or polypeptide; a primer usable for isolating a gene of a lachrymatory factor-producing enzyme of the genus allium and a method for isolating the gene with the primer; a recombinant vector containing the DNA; a transformant containing the recombinant vector containing the DNA; a method for preparing protein or a polypeptide having lachrymatory factor-producing enzymatic activity by culturing host cells transformed with the recombinant vector containing the DNA; protein or a polypeptide having the lachrymatory factor-producing enzymatic activity; and a nucleic acid molecule having a function of repressing the translation of mRNA on protein or polypeptide having the lachrymatory factor-prod

Abstract: The object of the present invention is to provide DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable.